产品: RPL3 抗体
货号: DF3706
描述: Rabbit polyclonal antibody to RPL3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 46 KD; 46kD(Calculated).
蛋白号: P39023
RRID: AB_2836070

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL3 Antibody detects endogenous levels of total RPL3.
RRID:
AB_2836070
引用格式: Affinity Biosciences Cat# DF3706, RRID:AB_2836070.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B; HIV1 TAR RNA binding protein B; MGC103388; MGC104284; MGC72934; Ribosomal protein L3; Ribosomal protein L3, isoform a; RL3_HUMAN; rpl3; TARBP B; TARBP-B;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL3, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P39023 作为底物

Site PTM Type Enzyme
S7 Phosphorylation
S13 Phosphorylation
R19 Methylation
K39 Ubiquitination
T54 Phosphorylation
S65 Phosphorylation
K66 Ubiquitination
K103 Acetylation
C114 S-Nitrosylation
K115 Acetylation
K115 Ubiquitination
K120 Ubiquitination
S125 Phosphorylation
K132 Ubiquitination
K136 Ubiquitination
K148 Acetylation
K148 Ubiquitination
K155 Ubiquitination
R169 Methylation
T189 Phosphorylation
K193 Ubiquitination
K224 Ubiquitination
K229 Ubiquitination
K250 Methylation
K250 Sumoylation
K250 Ubiquitination
S265 Phosphorylation
R268 Methylation
K286 Sumoylation
K286 Ubiquitination
Y291 Phosphorylation
K294 Acetylation
K294 Sumoylation
K294 Ubiquitination
K297 Ubiquitination
K300 Sumoylation
K300 Ubiquitination
S304 Phosphorylation
T305 Phosphorylation
Y307 Phosphorylation
S310 Phosphorylation
K312 Sumoylation
K312 Ubiquitination
K341 Acetylation
K341 Ubiquitination
K342 Acetylation
K349 Ubiquitination
S350 Phosphorylation
K356 Ubiquitination
K362 Acetylation
K362 Ubiquitination
K366 Acetylation
K366 Sumoylation
K366 Ubiquitination
K373 Acetylation
K373 Methylation
K373 Sumoylation
K373 Ubiquitination
T381 Phosphorylation
K385 Ubiquitination
K386 Sumoylation
K386 Ubiquitination
K393 Acetylation
K393 Sumoylation
K393 Ubiquitination
K394 Methylation
K399 Methylation
K399 Sumoylation

研究背景

功能:

The L3 protein is a component of the large subunit of cytoplasmic ribosomes.

细胞定位:

Nucleus>Nucleolus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the large ribosomal subunit (Probable). Interacts with METTL18. Interacts with DHX33.

蛋白家族:

Belongs to the universal ribosomal protein uL3 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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