产品: RPL15 抗体
货号: DF3698
描述: Rabbit polyclonal antibody to RPL15
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 20 KD; 24kD(Calculated).
蛋白号: P61313
RRID: AB_2836062

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL15 Antibody detects endogenous levels of total RPL15.
RRID:
AB_2836062
引用格式: Affinity Biosciences Cat# DF3698, RRID:AB_2836062.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L15; EC45; FLJ26304; ribosomal protein L15; RL15_HUMAN; RPL10; RPL15; RPLY10; RPYL10;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL15, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P61313 作为底物

Site PTM Type Enzyme
G2 Myristoylation
K5 Acetylation
K5 Ubiquitination
S34 Phosphorylation
K47 Ubiquitination
K54 Ubiquitination
K56 Ubiquitination
Y59 Phosphorylation
Y62 Phosphorylation
K77 Ubiquitination
Y81 Phosphorylation
K83 Acetylation
K83 Ubiquitination
K93 Acetylation
K93 Ubiquitination
S97 Phosphorylation
S100 Phosphorylation
R105 Methylation
T126 Phosphorylation
Y127 Phosphorylation
K140 Sumoylation
K140 Ubiquitination
K153 Acetylation
K153 Ubiquitination
K157 Ubiquitination
R162 Methylation
K179 Acetylation
K179 Ubiquitination
T183 Phosphorylation
S187 Phosphorylation
T197 Phosphorylation
R202 Methylation

研究背景

细胞定位:

Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with IFIT1 (via TPR repeats 1-4).

蛋白家族:

Belongs to the eukaryotic ribosomal protein eL15 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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