产品: RPL12 抗体
货号: DF3697
描述: Rabbit polyclonal antibody to RPL12
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 18 KD; 18kD(Calculated).
蛋白号: P30050
RRID: AB_2836061

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL12 Antibody detects endogenous levels of total RPL12.
RRID:
AB_2836061
引用格式: Affinity Biosciences Cat# DF3697, RRID:AB_2836061.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L12; L12; Ribosomal protein L12; RL12_HUMAN; rpl12;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL12, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECPAS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
78
Zebrafish
67
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30050 作为底物

Site PTM Type Enzyme
Y14 Phosphorylation
C17 S-Nitrosylation
K31 Ubiquitination
S38 Phosphorylation P24941 (CDK2)
K40 Acetylation
K40 Ubiquitination
K41 Ubiquitination
K48 Ubiquitination
K54 Acetylation
K54 Ubiquitination
K61 Ubiquitination
R67 Methylation
K83 Acetylation
K83 Ubiquitination
K99 Ubiquitination
S101 Phosphorylation
T105 Phosphorylation
R114 Methylation
S126 Phosphorylation
T128 Phosphorylation
K130 Ubiquitination
T135 Phosphorylation
S138 Phosphorylation
C141 S-Nitrosylation
C162 S-Nitrosylation
S165 Phosphorylation

研究背景

功能:

Binds directly to 26S ribosomal RNA.

蛋白家族:

Belongs to the universal ribosomal protein uL11 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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