产品: RPS8 抗体
货号: DF3689
描述: Rabbit polyclonal antibody to RPS8
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 30 KD; 24kD(Calculated).
蛋白号: P62241
RRID: AB_2836053

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
RPS8 Antibody detects endogenous levels of total RPS8.
RRID:
AB_2836053
引用格式: Affinity Biosciences Cat# DF3689, RRID:AB_2836053.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S8; OK/SW-cl.83; OTTHUMP00000010160; OTTHUMP00000010161; Ribosomal protein S8; RPS8; RS8_HUMAN; S8;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPS8, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62241 作为底物

Site PTM Type Enzyme
G2 Myristoylation
S4 Phosphorylation
K23 Acetylation
K24 Acetylation
K26 Sumoylation
K26 Ubiquitination
Y27 Phosphorylation
T36 Phosphorylation
K37 Ubiquitination
S69 Phosphorylation
C71 S-Nitrosylation
C72 S-Nitrosylation
T76 Phosphorylation
Y83 Phosphorylation
S86 Phosphorylation
T93 Phosphorylation
K98 Ubiquitination
C100 S-Nitrosylation
S106 Phosphorylation
T107 Phosphorylation
Y109 Phosphorylation
R110 Methylation
Y113 Phosphorylation
Y117 Phosphorylation
K125 Acetylation
K128 Acetylation
K128 Sumoylation
K128 Ubiquitination
T130 Phosphorylation
K139 Ubiquitination
S142 Phosphorylation
K157 Sumoylation
K157 Ubiquitination
S159 Phosphorylation
S160 Phosphorylation
K170 Ubiquitination
R184 Methylation
Y188 Phosphorylation
K193 Ubiquitination
Y198 Phosphorylation
R200 Methylation

研究背景

细胞定位:

Cytoplasm. Membrane>Lipid-anchor.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eS8 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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