ABCF1 Antibody - #AF0385

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产品: ABCF1 抗体
货号: AF0385
描述: Rabbit polyclonal antibody to ABCF1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 95kDa; 96kD(Calculated).
蛋白号: Q8NE71
RRID: AB_2833541

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
ABCF1 Antibody detects endogenous levels of total ABCF1.
RRID:
AB_2833541
引用格式: Affinity Biosciences Cat# AF0385, RRID:AB_2833541.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette, sub family F (GCN20), member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667;

抗原和靶标

免疫原:
Uniprot:

Q8NE71(ABCF1_HUMAN) >>Visit HPA database.

基因/基因ID:
ABCF1(23)
表达:
Q8NE71 ABCF1_HUMAN:

Ubiquitous.

描述:
ABCF1 May function in mRNA translation. Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Induced by TNF (in cultured synoviocytes). Interacts with eukaryotic initiation factor 2 (eIF2). Associates with the ribosomes. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
序列:
MPKAPKQQPPEPEWIGDGESTSPSDKVVKKGKKDKKIKKTFFEELAVEDKQAGEEEKVLKEKEQQQQQQQQQQKKKRDTRKGRRKKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAALDNEEEDKEEEIIKEKEPPKQGKEKAKKAEQGSEEEGEGEEEEEEGGESKADDPYAHLSKKEKKKLKKQMEYERQVASLKAANAAENDFSVSQAEMSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLKLLEEERRLQGQLEQGDDTAAERLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
91
Xenopus
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8NE71 作为底物

Site PTM Type Enzyme
K6 Ubiquitination
S20 Phosphorylation
T21 Phosphorylation
S22 Phosphorylation
S24 Phosphorylation
K26 Ubiquitination
K39 Ubiquitination
K50 Ubiquitination
S105 Phosphorylation
T108 Phosphorylation
S109 Phosphorylation P68400 (CSNK2A1)
S140 Phosphorylation P68400 (CSNK2A1)
K163 Acetylation
K163 Methylation
S166 Phosphorylation
K174 Methylation
K174 Ubiquitination
K176 Methylation
K209 Ubiquitination
S228 Phosphorylation
S244 Phosphorylation
Y250 Phosphorylation
S254 Phosphorylation
K255 Ubiquitination
K263 Ubiquitination
Y267 Phosphorylation
S273 Phosphorylation
K275 Methylation
K275 Ubiquitination
S287 Phosphorylation
K305 Ubiquitination
K308 Ubiquitination
Y332 Phosphorylation
T343 Phosphorylation
T344 Phosphorylation
K347 Ubiquitination
K389 Ubiquitination
T407 Phosphorylation
K414 Ubiquitination
Y416 Phosphorylation
T422 Phosphorylation
K430 Ubiquitination
K451 Ubiquitination
K537 Methylation
K549 Ubiquitination
Y551 Phosphorylation
K573 Ubiquitination
K576 Ubiquitination
T580 Phosphorylation
K582 Ubiquitination
K589 Ubiquitination
S595 Phosphorylation
K603 Ubiquitination
S621 Phosphorylation
S652 Phosphorylation
S665 Phosphorylation
T666 Phosphorylation
T672 Phosphorylation
T676 Phosphorylation
T678 Phosphorylation
K689 Ubiquitination
Y710 Phosphorylation
K748 Ubiquitination

研究背景

功能:

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

翻译修饰:

Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.

细胞定位:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.

蛋白家族:

Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.

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