HSD3B7 Antibody - #DF3653

Figure 5. Overexpression of miR-7481-3p promotes testosterone synthesis in rooster Leydig cells. (A) Treatment with miR-7481-3p mimics for 48 h increases the relative mRNA expression of miR-7481-3p in roosters Leydig cells. (B) The level of testosterone in rooster Leydig cells treated with miR-7481-3p mimics for 48 h. (C–H) Treatment with miR-7481-3p mimics for 48 h increases the relative mRNA and protein expression of StAR, CYP11A1, and 3β-HSD in rooster Leydig cells. All data are presented as the mean ± SD (n = 3), * p < 0.05, ** p < 0.01 and *** p < 0.001.

Figure 5. Inhibited Drp1 and PINK1 expression reduces mitophagy and steroid hormones synthesis in granulosa cells under acute heat treatment. (A, C) Western blot results showing the protein (LC3-II/LC3-I, Beclin1, p62, PINK1, Tom20) level in granulosa cells transfected with si-Drp1 and si-PINK1. (B, D) Western blot results showing the protein (StAR, CYP11A1, 3β-HSD, CYP19A1) level in granulosa cells transfected with si-Drp1 and si-PINK1. β-Actin was used as an internal reference in whole-cell lysates. Each experiment was repeated 3 times. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01.