| 产品: | ORC1L 抗体 |
| 货号: | DF3637 |
| 描述: | Rabbit polyclonal antibody to ORC1L |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Rabbit |
| 分子量: | 100,120 KD; 97kD(Calculated). |
| 蛋白号: | Q13415 |
| RRID: | AB_2836009 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3637, RRID:AB_2836009.
展开/折叠
HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 (yeast homolog) like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like (yeast); PARC1; Replication control protein 1;
抗原和靶标
- Q13415 ORC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSSSDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRNDLLLRVRLNVSQDDVLYALKDE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13415 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K25 | Sumoylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S269 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S275 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| K326 | Acetylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K364 | Acetylation | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| S383 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| K448 | Methylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| K859 | Ubiquitination | Uniprot |
研究背景
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Phosphorylated during mitosis.
Nucleus.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated.
The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.
Belongs to the ORC1 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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