| 产品: | PARP2 抗体 |
| 货号: | DF3625 |
| 描述: | Rabbit polyclonal antibody to PARP2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 75 KD; 66kD(Calculated). |
| 蛋白号: | Q9UGN5 |
| RRID: | AB_2835997 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3625, RRID:AB_2835997.
展开/折叠
ADP ribosyltransferase like 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT 2; ADPRT-2; ADPRT2; ADPRTL 2; ADPRTL 3; ADPRTL2; ADPRTL3; ARTD2; hPARP 2; hPARP-2; hPARP2; NAD(+) ADP ribosyltransferase 2; NAD(+) ADP-ribosyltransferase 2; pADPRT 2; pADPRT-2; pADPRT2; PARP 2; PARP-2; PARP2; PARP2_HUMAN; Poly (ADP ribose) polymerase family member 2; Poly (ADP ribosyl) transferase like 2; poly (ADP-ribose) polymerase 2; Poly [ADP ribose] synthetase 2; Poly [ADP-ribose] polymerase 2; Poly(ADP ribose) synthetase; Poly[ADP-ribose] synthase 2;
抗原和靶标
Widely expressed, mainly in actively dividing tissues (PubMed:10364231). The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus (PubMed:10364231).
- Q9UGN5 PARP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.
Poly-ADP-ribosylated by PARP1.
Acetylation reduces DNA binding and enzymatic activity.
Nucleus.
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LRIG3 (By similarity). Homo- and heterodimer with PARP1. Interacts weakly (via the PARP catalytic domain) with HPF1.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Genetic Information Processing > Replication and repair > Base excision repair.
限制条款
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