| 产品: | BARD1 抗体 |
| 货号: | DF3605 |
| 描述: | Rabbit polyclonal antibody to BARD1 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 分子量: | 79 KD; 87kD(Calculated). |
| 蛋白号: | Q99728 |
| RRID: | AB_2835977 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3605, RRID:AB_2835977.
展开/折叠
BARD-1; Bard1; BARD1_HUMAN; BRCA1 associated RING domain 1; BRCA1 associated RING domain gene 1; BRCA1 associated RING domain protein 1; BRCA1-associated RING domain protein 1;
抗原和靶标
- Q99728 BARD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPDNRQPRNRQPRIRSGNEPRSAPAMEPDGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSMIQLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKWNLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLTEVSLPLAEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSILSTSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLSSSSYRRVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGKVWKAPSSWFIDCVMSFELLPLDS
翻译修饰 - Q99728 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K46 | Sumoylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K124 | Acetylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K144 | Sumoylation | Uniprot | |
| S148 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| K152 | Acetylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K170 | Sumoylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K217 | Sumoylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S288 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| T299 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| K305 | Sumoylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K423 | Sumoylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| K548 | Sumoylation | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| T719 | Phosphorylation | Uniprot | |
| T734 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
Nucleus.
Note: During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.