| 产品: | Cytochrome P450 1A2 抗体 |
| 货号: | DF3566 |
| 描述: | Rabbit polyclonal antibody to Cytochrome P450 1A2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 55 KD; 58kD(Calculated). |
| 蛋白号: | P05177 |
| RRID: | AB_2835938 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3566, RRID:AB_2835938.
展开/折叠
Aryl hydrocarbon hydroxylase; CP 12; CP12; CP1A2_HUMAN; CYP1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 1A2; Cytochrome P450 4; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 family 1 subfamily A polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cytochrome P450-P3; Cytochrome P4501A2; Dioxin inducable P3 450; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P(3)450; P3 450; P450 4; P450 form 4; P450 P3; P450(PA); Xenobiotic monooxygenase;
抗原和靶标
- P05177 CP1A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARLRFSIN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05177 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y72 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| T438 | Phosphorylation | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation (Probable).
Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.
Liver.
Belongs to the cytochrome P450 family.
研究领域
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Lipid metabolism > Linoleic acid metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.
· Metabolism > Global and overview maps > Metabolic pathways.
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