Collagen VI alpha 2 Antibody - #DF3552

Fig. 5|The diferentially expressed proteins were validated by Western Blot. Compared with adjacent normal tissues, the protein expression of PTMA, PAK2, PPP1CA, HMGB2 were up-regulated (a, b), and the protein expression of Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1, Vinculin were down-regulated in ESCC tissues from four pairs of samples (c, d). Representative immunoblot images (a, c) and histograms (mean ± SD; b, d).The experiments were repeated at least three times, N represented normal tissues and T represented tumor tissues

Figure3 ITGA6 was downregulated, Col6α1 and Col6α2 were upregulated in the testis of the model group exposed to an estrogen-enriched diet and cold for 24 weeks. (a) Immunofluorescence staining for ITGA6 (red, 200×), Col6α1 (green, 100×), Col6α2 (red, 100×); nuclei were stained with DAPI (blue). (b, c, and d) Representative western blot results for ITGA6, Col6α1, Col6α2. (e, f, and g) Relative expression of ITGA6, Col6α1, Col6α2 compared with GAPDH. Protein lysates were obtained from the testis of either model (M) or control rat group (N). Protein expression of ITGA6, Col6α1, and Col6α2 was evaluated by western blotting using specific antibodies for the target proteins, and the signal was then quantified using Image J. (h, i, and j) qRT-PCR of ITGA6, Col6α1, and Col6α2 in the testis of rats in the two groups. Values are expressed as the mean ± SD. Statistical significance is indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to the control group.

Figure3 ITGA6 was downregulated, Col6α1 and Col6α2 were upregulated in the testis of the model group exposed to an estrogen-enriched diet and cold for 24 weeks. (a) Immunofluorescence staining for ITGA6 (red, 200×), Col6α1 (green, 100×), Col6α2 (red, 100×); nuclei were stained with DAPI (blue). (b, c, and d) Representative western blot results for ITGA6, Col6α1, Col6α2. (e, f, and g) Relative expression of ITGA6, Col6α1, Col6α2 compared with GAPDH. Protein lysates were obtained from the testis of either model (M) or control rat group (N). Protein expression of ITGA6, Col6α1, and Col6α2 was evaluated by western blotting using specific antibodies for the target proteins, and the signal was then quantified using Image J. (h, i, and j) qRT-PCR of ITGA6, Col6α1, and Col6α2 in the testis of rats in the two groups. Values are expressed as the mean ± SD. Statistical significance is indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to the control group.