Collagen IV alpha 2 Antibody - #DF3550

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产品: Collagen IV alpha 2 抗体
货号: DF3550
描述: Rabbit polyclonal antibody to Collagen IV alpha 2
应用: WB IHC IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit
蛋白号: P08572
RRID: AB_2835923

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Collagen IV alpha 2 Antibody detects endogenous levels of total Collagen IV alpha 2.
RRID:
AB_2835923
引用格式: Affinity Biosciences Cat# DF3550, RRID:AB_2835923.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Canstatin; CO4A2_HUMAN; COL4A 2; Col4a2; Collagen alpha 2(IV) chain; Collagen, type IV, alpha 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human Collagen IV alpha 2, corresponding to a region within N-terminal amino acids.

基因/基因ID:
COL4A2(1284)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Canstatin represses glioma growth by inhibiting formation of VM-like structures. Translational Neuroscience, 2021 (PubMed: 34434564) [IF=1.8]

Application: IF/ICC    Species: Mice    Sample: U87 cell

Figure 1 Vascular tube formation in vitro. Fluorescent images of U87 (a) and U251 (c) cells transferred with pCDH-EFGP and pCDH-Canstatin-EGFP. Scar bars, 50 μm. Quantification of mRNA expression levels of canstatin in U87 (b) and U215 (d) cells. Tube formation was measured with U87 (e) and U251 (f) cells expressing lentiviral pCDH vector or pCDH-canstatin. Tubules were imaged using phase contrast. A representative of three to five fields is shown. Scale bars, 20 μm. Quantification of tubules from three to five random fields in each well planted with the same amounts of U87 (g) and U251 (h) cells. Values are represented as means ± SD, n = 4 each group, *p < 0.05, **p < 0.01, and ***p < 0.005.

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