CDCA2 Antibody - #DF3543

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产品: CDCA2 抗体
货号: DF3543
描述: Rabbit polyclonal antibody to CDCA2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 113,140kD; 113kD(Calculated).
蛋白号: Q69YH5
RRID: AB_2835916

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
CDCA2 Antibody detects endogenous levels of total CDCA2.
RRID:
AB_2835916
引用格式: Affinity Biosciences Cat# DF3543, RRID:AB_2835916.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cell division cycle associated 2; PPP1R81; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man; protein phosphatase 1, regulatory subunit 81; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo Man; Repo-Man;

抗原和靶标

免疫原:
Uniprot:

Q69YH5(CDCA2_HUMAN) >>Visit HPA database.

基因/基因ID:
CDCA2(157313)
表达:
Q69YH5 CDCA2_HUMAN:

Ubiquitously expressed.

序列:
MDANSKDKPPETKESAMNNAGNASFILGTGKIVTPQKHAELPPNPCTPDTFKSPLNFSTVTVEQLGITPESFVRNSAGKSSSYLKKCRRRSAVGARGSPETNHLIRFIARQQNIKNARKSPLAQDSPSQGSPALYRNVNTLRERISAFQSAFHSIKENEKMTGCLEFSEAGKESEMTDLTRKEGLSACQQSGFPAVLSSKRRRISYQRDSDENLTDAEGKVIGLQIFNIDTDRACAVETSVDLSEISSKLGSTQSGFLVEESLPLSELTETSNALKVADCVVGKGSSDAVSPDTFTAEVSSDAVPDVRSPATPACRRDLPTPKTFVLRSVLKKPSVKMCLESLQEHCNNLYDDDGTHPSLISNLPNCCKEKEAEDEENFEAPAFLNMRKRKRVTFGEDLSPEVFDESLPANTPLRKGGTPVCKKDFSGLSSLLLEQSPVPEPLPQPDFDDKGENLENIEPLQVSFAVLSSPNKSSISETLSGTDTFSSSNNHEKISSPKVGRITRTSNRRNQLVSVVEESVCNLLNTEVQPCKEKKINRRKSQETKCTKRALPKKSQVLKSCRKKKGKGKKSVQKSLYGERDIASKKPLLSPIPELPEVPEMTPSIPSIRRLGSGYFSSNGKLEEVKTPKNPVKRKDLLRHDPDLHMHQGYDKYDVSEFCSYIKSSSSLGNATSDEDPNTNIMNINENKNIPKAKNKSESENEPKAGTDSPVSCASVTEERVASDSPKPALTLQQGQEFSAGGQNAENLCQFFKISPDLNIKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSEDLGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSSSVGSSVEISLENSELFKDLSDAIEQTFQRRNSETKVRRSTRLQKDLENEGLVWISLPLPSTSQKAKRRTICTFDSSGFESMSPIKETVSSRQKPQMAPPVSDPENSQGPAAGSSDEPGKRRKSFCISTLANTKATSQFKGYRRRSSLNGKGESSLTALERIEHNGERKQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
100
Bovine
91
Sheep
91
Dog
91
Horse
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.

翻译修饰:

Phosphorylated by CDK1. May regulate its subcellular location.

细胞定位:

Nucleus.
Note: Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Interacts with PPP1CC.

文献引用

1). Cell division cycle associated 2 (CDCA2) upregulation promotes the progression of hepatocellular carcinoma in a p53-dependant manner. PeerJ, 2017 (PubMed: 35694386) [IF=2.3]

Application: WB    Species: Human    Sample: liver tissues

Figure 1 Expression of CDCA2 in hepatocellular carcinoma. Analysis of an RNA-seq dataset from UALCAN showed (A) elevated CDCA2 expression in primary HCC tissues compared with normal liver tissues; (B) increased CDCA2 levels with clinical stage; (C) elevated CDCA2 expression in samples expressing mutant TP53 compared with samples expressing wild-type TP53; (D) worse survival of patients with high CDCA2 expression than in patients with low CDCA2 expression; and (E) worse survival of patients with high CDCA2 expression than patients with low CDCA2 expression among Asian patients but not among White Caucasian patients. (F) Our study found elevated CDCA2 mRNA levels in 40 HCC tissues compared to paired normal liver tissues. (G) Representative photos of +ve and –ve immunohistochemistry staining of CDCA2 in HCC or normal liver tissues. (H) qRT-PCR measured CDCA2 mRNA expression in the L02 and HCC cell lines. (I) Western blotting analysis of CDCA2 protein expression in the L02 and HCC cell lines. Stars indicate the P value of the CDCA2 protein level compared with that in the L02 cells. (J) Western blotting analysis of CDCA2 protein expression in HCC cell lines with lentivirus-mediated stable CDCA2 knockdown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

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