RPS19 Antibody - #DF3680

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产品: RPS19 抗体
货号: DF3680
描述: Rabbit polyclonal antibody to RPS19
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
分子量: 16 KD; 16kD(Calculated).
蛋白号: P39019
RRID: AB_2835890

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
RPS19 Antibody detects endogenous levels of total RPS19.
RRID:
AB_2835890
引用格式: Affinity Biosciences Cat# DF3680, RRID:AB_2835890.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S19; DBA; DBA1; HGNC:10402; Ribosomal protein S19; rps19; RS19_HUMAN; S19;

抗原和靶标

免疫原:
Uniprot:

P39019(RS19_HUMAN) >>Visit HPA database.

基因/基因ID:
RPS19(6223)
表达:
P39019 RS19_HUMAN:

Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.

序列:
MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
82
Xenopus
73
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P39019 作为底物

Site PTM Type Enzyme
K7 Ubiquitination
K23 Acetylation
K29 Ubiquitination
K38 Ubiquitination
K43 Ubiquitination
Y48 Phosphorylation
Y54 Phosphorylation
T55 Phosphorylation
R56 Methylation
S59 Phosphorylation P17612 (PRKACA) , Q14012 (CAMK1)
R67 Methylation
S74 Phosphorylation
T76 Phosphorylation
K77 Acetylation
K77 Ubiquitination
R94 Methylation
K111 Acetylation
K111 Ubiquitination
K115 Acetylation
K122 Ubiquitination
R133 Methylation
K143 Acetylation
K143 Ubiquitination
K144 Acetylation

研究背景

功能:

Required for pre-rRNA processing and maturation of 40S ribosomal subunits.

细胞定位:

Nucleus.
Note: Located more specifically in the nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.

亚基结构:

Interacts with RPS19BP1.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eS19 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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