产品: THRB 抗体
货号: AF0357
描述: Rabbit polyclonal antibody to THRB
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
分子量: 70kDa; 70kD(Calculated).
蛋白号: P00734
RRID: AB_2833522

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(82%), Bovine(82%), Horse(82%), Sheep(82%), Dog(91%)
克隆:
Polyclonal
特异性:
THRB Antibody detects endogenous levels of total THRB.
RRID:
AB_2833522
引用格式: Affinity Biosciences Cat# AF0357, RRID:AB_2833522.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

coagulation factor II (thrombin); Coagulation factor II; F2; Factor II; Prepro coagulation factor II; Prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; THRB; THRB_HUMAN; Thrombin heavy chain;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P00734 THRB_HUMAN:

Expressed by the liver and secreted in plasma.

描述:
prothrombin Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Belongs to the peptidase S1 family.
序列:
MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Dog
91
Horse
82
Bovine
82
Sheep
82
Zebrafish
82
Xenopus
78
Chicken
70
Rabbit
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00734 作为底物

Site PTM Type Enzyme
N121 N-Glycosylation
N143 N-Glycosylation
S199 Phosphorylation
Y320 Phosphorylation
S331 Phosphorylation
N416 N-Glycosylation
Y434 Phosphorylation
S539 Phosphorylation
T540 Phosphorylation
K599 Acetylation

研究背景

功能:

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

翻译修饰:

The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.

N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

细胞定位:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed by the liver and secreted in plasma.

亚基结构:

Heterodimer (named alpha-thrombin) of a light and a heavy chain; disulfide-linked. Forms a heterodimer with SERPINA5.

蛋白家族:

Belongs to the peptidase S1 family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

文献引用

1). Wang R et al. KMT2D Regulates the NCOA6/THRB Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer. Frontiers in Bioscience - Elite 2023 Jan 18;28(1):17. (PubMed: 36722273)

Application: WB    Species: Human    Sample: thyroid papillary carcinoma cells

Fig. 3. The mechanism of KMT2D on papillary thyroid carcinoma cells. (a) ChIP assay. (b) qRT-PCR analysis. (c) Western blotting analysis. A, si-NC; B, si-KMT2D (1); C, si- KMT2D (1).

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