PTTG1 Antibody - #AF0354

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产品: PTTG1 抗体
货号: AF0354
描述: Rabbit polyclonal antibody to PTTG1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit, Dog
蛋白号: O95997
RRID: AB_2833520

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
PTTG1 Antibody detects endogenous levels of total PTTG1.
RRID:
AB_2833520
引用格式: Affinity Biosciences Cat# AF0354, RRID:AB_2833520.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AW555095; C87862; Cut2; EAP 1; EAP1; ESP1 associated protein 1; Esp1-associated protein; hPTTG; MGC126883; MGC138276; Pds1; Pituitary tumor transforming 1; Pituitary tumor transforming protein 1; Pituitary tumor-transforming 1, isoform CRA_a; Pituitary tumor-transforming 1, isoform CRA_b; Pituitary tumor-transforming gene 1; Pituitary tumor-transforming gene 1 protein; PTTG 1; PTTG; PTTG1; PTTG1 protein; PTTG1_HUMAN; Pttg3; Securin; Tumor transforming 1; Tumor transforming protein 1; Tumor-transforming protein 1; TUTR 1; TUTR1;

抗原和靶标

免疫原:

A synthesized peptide derived from human PTTG1, corresponding to a region within the internal amino acids.

基因/基因ID:
PTTG1(9232)
描述:
securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

文献引用

1). Comparison of the major cell populations among osteoarthritis, Kashin–Beck disease and healthy chondrocytes by single-cell RNA-seq analysis. Cell Death & Disease, 2021 (PubMed: 34045450) [IF=8.1]

Application: IHC    Species: Human    Sample: cartilage tissues

Fig. 4 Single-cell RNA-sequencing analysis of the cell population in healthy controls. A Gene expression plots of CENPW, PTTG1 and BIRC5. Dot colour corresponds to the level of gene expression in each cell. t-SNE, t-distributed stochastic neighbour embedding. B Representative immunohistochemistry staining of CENPW and PTTG1 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (up, middle, deep) in healthy cartilage tissues displayed by box plot (n = 5). *p < 0.05. C, D Monocle pseudotime trajectory showing the progression of KBD, OA and the control. E The expression of the genes in a branch-dependent manner. Each row indicates the standardised kinetic curves of a gene. The centre of the heatmap shows the kinetic curve value at the root of the trajectory. From the centre to the left of the heatmap, the kinetic curve progresses from the root along the trajectory to fate 1. Starting from the right, the curve from the root to fate 2.

Application: IHC    Species: human    Sample: cartilage

Fig. 4 | Single-cell RNA-sequencing analysis of the cell population in healthy controls.B Representative immunohistochemistry staining of CENPW and PTTG1 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (up, middle, deep) in healthy cartilage tissues displayed by box plot (n = 5). *p < 0.05.
2). Integrated analysis of single-cell and bulk RNA sequencing data reveals a cellular senescence-related signature in hepatocellular carcinoma. Frontiers in cell and developmental biology, 2024 (PubMed: 38887516) [IF=4.6]
3). SIRT1 suppresses pituitary tumor progression by downregulating PTTG1 expression. ONCOLOGY REPORTS, 2022 (PubMed: 35730625) [IF=3.8]

Application: WB    Species: Human    Sample:

Figure 5. - H3K9ac reader BRD4 is required for PTTG1 expression. (A) Bar plot revealing the relative mRNA expression. *P

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