DBF4 Antibody - #DF3462

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产品: DBF4 抗体
货号: DF3462
描述: Rabbit polyclonal antibody to DBF4
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Horse, Dog
分子量: 77 KD; 77kD(Calculated).
蛋白号: Q9UBU7
RRID: AB_2835826

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(86%), Horse(83%), Dog(86%)
克隆:
Polyclonal
特异性:
DBF4 Antibody detects endogenous levels of total DBF4.
RRID:
AB_2835826
引用格式: Affinity Biosciences Cat# DF3462, RRID:AB_2835826.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4 homolog; DBF4 type zinc finger containing protein 1; DBF4 zinc finger A; DBF4 zinc finger; DBF4, S. cerevisiae, homolog of; DBF4-type zinc finger-containing protein 1; DBF4A; DBF4A_HUMAN; Dumbbell former 4, S. cerevisiae, homolog of; OTTHUMP00000161661; Protein DBF4 homolog A; ZDBF1; Zinc finger, DBF-type containing 1;

抗原和靶标

免疫原:
Uniprot:

Q9UBU7(DBF4A_HUMAN) >>Visit HPA database.

基因/基因ID:
DBF4(10926)
表达:
Q9UBU7 DBF4A_HUMAN:

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

序列:
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
86
Dog
86
Horse
83
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBU7 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
K21 Sumoylation
K81 Ubiquitination
T98 Phosphorylation
S103 Phosphorylation
S107 Phosphorylation
S130 Phosphorylation
K140 Ubiquitination
Y177 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
K201 Ubiquitination
S260 Phosphorylation
T273 Phosphorylation
Y278 Phosphorylation
T281 Phosphorylation
S282 Phosphorylation
Y303 Phosphorylation
S312 Phosphorylation
T345 Phosphorylation
Y353 Phosphorylation
S354 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S362 Phosphorylation
S364 Phosphorylation
S381 Phosphorylation
K393 Ubiquitination
K408 Sumoylation
S413 Phosphorylation
S420 Phosphorylation
K429 Ubiquitination
S435 Phosphorylation
T449 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
K455 Ubiquitination
S465 Phosphorylation
T468 Phosphorylation
S470 Phosphorylation
S502 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
S508 Phosphorylation
T510 Phosphorylation
T535 Phosphorylation
S539 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
T553 Phosphorylation
S568 Phosphorylation
S571 Phosphorylation
K579 Sumoylation
S612 Phosphorylation
S613 Phosphorylation
S629 Phosphorylation
T635 Phosphorylation
T638 Phosphorylation
S664 Phosphorylation
S665 Phosphorylation

研究背景

功能:

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

翻译修饰:

Phosphorylation increases its interaction with PSIP1.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

亚基结构:

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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