IARS2 Antibody - #DF3448

(2)  
产品: IARS2 抗体
货号: DF3448
描述: Rabbit polyclonal antibody to IARS2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 114 KD; 114kD(Calculated).
蛋白号: Q9NSE4
RRID: AB_2835814

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(90%), Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
IARS2 Antibody detects endogenous levels of total IARS2.
RRID:
AB_2835814
引用格式: Affinity Biosciences Cat# DF3448, RRID:AB_2835814.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2010002H18Rik; C79125; EC 6.1.1.5; FLJ10326; Iars2; IleRS; isoleucine tRNA ligase 2, mitochondrial; isoleucine tRNA ligase, mitochondrial; isoleucine tRNA synthetase 2, mitochondrial; Isoleucine--tRNA ligase; isoleucine-tRNA synthetase 2; isoleucyl tRNA synthetase 2, mitochondrial; Isoleucyl-tRNA synthetase; MGC63429; mitochondrial; mitochondrial isoleucine tRNA synthetase; rCG_20084; RGD1311857; SYIM_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q9NSE4(SYIM_HUMAN) >>Visit HPA database.

基因/基因ID:
IARS2(55699)
序列:
MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
90
Bovine
90
Chicken
78
Zebrafish
75
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NSE4 作为底物

Site PTM Type Enzyme
S44 Phosphorylation
S46 Phosphorylation
S57 Phosphorylation
Y60 Phosphorylation
K133 Ubiquitination
S186 Phosphorylation
K189 Acetylation
K189 Ubiquitination
K222 Acetylation
R225 Methylation
Y231 Phosphorylation
K233 Acetylation
Y237 Phosphorylation
K241 Acetylation
K241 Methylation
K241 Ubiquitination
T251 Phosphorylation
Y260 Phosphorylation
K445 Ubiquitination
K455 Sumoylation
K458 Ubiquitination
K479 Acetylation
K493 Ubiquitination
K500 Acetylation
K540 Ubiquitination
Y543 Phosphorylation
S547 Phosphorylation
Y621 Phosphorylation
T657 Phosphorylation
K687 Ubiquitination
K725 Acetylation
K775 Acetylation
K781 Acetylation
Y798 Phosphorylation
K803 Acetylation
K872 Ubiquitination
S881 Phosphorylation
S888 Phosphorylation
K896 Ubiquitination
T950 Phosphorylation
T982 Phosphorylation
C1005 S-Nitrosylation

研究背景

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the class-I aminoacyl-tRNA synthetase family.

研究领域

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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