BCA3 Antibody - #DF3444

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产品: BCA3 抗体
货号: DF3444
描述: Rabbit polyclonal antibody to BCA3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: Q9NQ31
RRID: AB_2835810

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
BCA3 Antibody detects endogenous levels of total BCA3.
RRID:
AB_2835810
引用格式: Affinity Biosciences Cat# DF3444, RRID:AB_2835810.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AKIP1; BCA3; Breast cancer associated gene 3 protein; Chromosome 11 open reading frame 17; Koyt binding protein; PKA interacting protein; Proline rich protein BCA3; Protein kinase A interacting protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human BCA3, corresponding to a region within the internal amino acids.

基因/基因ID:
AKIP1(56672)

文献引用

1). UBE2S facilitates glioblastoma progression through activation of the NF-κB pathway via attenuating K11-linked ubiquitination of AKIP1. International journal of biological macromolecules, 2024 (PubMed: 39098687) [IF=7.7]

Application: WB    Species: human    Sample:

Fig. 4. UBE2S upregulates the protein expression of AKIP1. (A) The protein expression of AKIP1 in human astrocyte (NHA) and various GBM cell lines (A172, U251, U87, T98G, LN319). (B) Bar chart of relative protein expression of AKIP1 in the indicated cell lines. (C) The protein expression of AKIP1 at different time points after CHX treatment in shNC and shUBE2S cell lines and statistical chart (D). (E) Western blotting of Flag-AKIP1 in different groups as indicated. (F) Bar chart of relative protein expression of AKIP1. (G) AKIP1 protein levels changes as UBE2S knocking down in U87 MG cells as indicated by western blotting. (H) Bar chart of relative protein expression of UBE2S in U87 MG cells. (I) Bar chart of relative protein expression of AKIP1 in U87 MG cells. (J) AKIP1 protein levels changes as UBE2S knocking down in U251 MG cells as indicated by western blotting. (K) Bar chart of relative protein expression of UBE2S in U251 MG cells. (L) Bar chart of relative protein expression of AKIP1 in U251 MG cells. (* P < 0.05, *** P < 0.001).

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