产品: MED24 抗体
货号: AF0346
描述: Rabbit polyclonal antibody to MED24
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 110kDa; 110kD(Calculated).
蛋白号: O75448
RRID: AB_2833516

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
MED24 Antibody detects endogenous levels of total MED24.
RRID:
AB_2833516
引用格式: Affinity Biosciences Cat# AF0346, RRID:AB_2833516.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CRSP complex subunit 4; Activator recruited cofactor 100 kDa component; Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; hTRAP100; KIAA0130; MED 24; MED24; MED24_HUMAN; Mediator complex subunit 24; Mediator of RNA polymerase II transcription subunit 24; Mediator of RNA polymerase II transcription subunit 24 homolog; MGC8748; OTTHUMP00000164458; OTTHUMP00000164460; THRAP4; Thyroid hormone receptor associated protein 4; Thyroid hormone receptor associated protein complex 100 kDa component; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; Trapp100; Vitamin D3 receptor interacting protein complex component DRIP100; Vitamin D3 receptor-interacting protein complex 100 kDa component;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O75448 MED24_HUMAN:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

描述:
Trap100 a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors.
序列:
MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLADALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAERLREGLEAGTPAAGEKQLAMCLQRLEKTLSSTKNRALLHIAKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHAEQMHKTGFPTVHAVILLEGTMNLTGETQSLVEQLTMVKRMQHIPTPLFVLEIWKACFVGLIESPEGTEELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRCNCDCTNFLLQECGKQGLLSEASVNNLMAKRKADREHAPQQKSGENANIQPNIQLILRAEPTVTNILKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTYGSEESTKPASVRALLFDISFLMLCHVAQTYGSEVILSESRTGAEVPFFETWMQTCMPEEGKILNPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFESIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLFSENTLQFYNERVVIMNSILERMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLEKGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVTLVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASTRQKKRHREDIEDYISLFPLDDVQPSKLMRLLSSNEDDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRVLANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGGRGSVLQFMPFTTVSELVKVSAMSSPKVVLAITDLSLPLGRQVAAKAIAAL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
91
Xenopus
91
Zebrafish
91
Rabbit
91
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75448 作为底物

Site PTM Type Enzyme
T36 Phosphorylation
Y66 Phosphorylation
Y75 Phosphorylation
K156 Ubiquitination
K167 Ubiquitination
T172 Phosphorylation
K350 Ubiquitination
T360 Phosphorylation
S374 Phosphorylation
S377 Phosphorylation
K384 Ubiquitination
S397 Phosphorylation
K422 Ubiquitination
T423 Phosphorylation
S429 Phosphorylation
S444 Phosphorylation
S447 Phosphorylation
K460 Ubiquitination
K484 Ubiquitination
K638 Ubiquitination
S652 Phosphorylation
T655 Phosphorylation
K717 Ubiquitination
K721 Ubiquitination
K856 Ubiquitination
S862 Phosphorylation
S863 Phosphorylation
S872 Phosphorylation
S873 Phosphorylation
T875 Phosphorylation
K965 Ubiquitination
T971 Phosphorylation
K984 Ubiquitination

研究背景

功能:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

亚基结构:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR.

蛋白家族:

Belongs to the Mediator complex subunit 24 family.

研究领域

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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