产品: BLZF1 抗体
货号: DF3432
描述: Rabbit polyclonal antibody to BLZF1
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 47 KD; 45kD(Calculated).
蛋白号: Q9H2G9
RRID: AB_2835732

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
BLZF1 Antibody detects endogenous levels of total BLZF1.
RRID:
AB_2835732
引用格式: Affinity Biosciences Cat# DF3432, RRID:AB_2835732.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1700030G05Rik; basic leucine zipper nuclear factor 1 (JEM 1); Basic leucine zipper nuclear factor 1; BLZF1; Blzf1l; GO45_HUMAN; GOLGIN 45; Golgin-45; JEM 1; JEM 1s; JEM-1; JEM1; MGC22497; p45 basic leucine zipper nuclear factor; p45 basic leucine-zipper nuclear factor;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9H2G9 GO45_HUMAN:

Detected in adrenal gland (PubMed:9129147).

序列:
MTTKNLETKVTVTSSPIRGAGDGMETEEPPKSVEVTSGVQSRKHHSLQSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Chicken
86
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

翻译修饰:

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.

细胞定位:

Golgi apparatus membrane.

Nucleus.
Note: Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm.

Cytoplasm.
Note: Not detected in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in adrenal gland.

亚基结构:

Interacts with GORASP2. Interacts with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. Identified in a complex with RAB2 and GORASP2.

蛋白家族:

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.

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