MAP4K3 Antibody - #DF3342

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产品: MAP4K3 抗体
货号: DF3342
描述: Rabbit polyclonal antibody to MAP4K3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Dog
分子量: 101 KD; 101kD(Calculated).
蛋白号: Q8IVH8
RRID: AB_2835709

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MAP4K3 Antibody detects endogenous levels of total MAP4K3.
RRID:
AB_2835709
引用格式: Affinity Biosciences Cat# DF3342, RRID:AB_2835709.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Germinal center kinase like kinase; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1;

抗原和靶标

免疫原:
Uniprot:

Q8IVH8(M4K3_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP4K3(8491)
表达:
Q8IVH8 M4K3_HUMAN:

Ubiquitously expressed in all tissues examined, with high levels in heart, brain, placenta, skeletal muscle, kidney and pancreas and lower levels in lung and liver.

序列:
MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8IVH8 作为底物

Site PTM Type Enzyme
M1 Acetylation
S24 Phosphorylation
T26 Phosphorylation
Y27 Phosphorylation
Y31 Phosphorylation
K32 Ubiquitination
T145 Phosphorylation
S158 Phosphorylation
T164 Phosphorylation
K167 Ubiquitination
S170 Phosphorylation Q8IVH8 (MAP4K3)
S280 Phosphorylation
T327 Phosphorylation
S329 Phosphorylation
Y366 Phosphorylation
Y367 Phosphorylation
Y379 Phosphorylation
Y387 Phosphorylation
K397 Ubiquitination
S398 Phosphorylation
S422 Phosphorylation
S467 Phosphorylation
S507 Phosphorylation
K530 Acetylation
S533 Phosphorylation
T539 Phosphorylation
K541 Acetylation
S616 Phosphorylation
S618 Phosphorylation
S661 Phosphorylation
Y735 Phosphorylation
S743 Phosphorylation
S893 Phosphorylation

翻译修饰 - Q8IVH8 作为激酶

Substrate Site Source
Q04759 (PRKCQ) T538 Uniprot
Q8IVH8 (MAP4K3) S170 Uniprot

研究背景

功能:

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.

组织特异性:

Ubiquitously expressed in all tissues examined, with high levels in heart, brain, placenta, skeletal muscle, kidney and pancreas and lower levels in lung and liver.

亚基结构:

Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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