TNFAIP8L2 Antibody - #DF3326

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产品: TNFAIP8L2 抗体
货号: DF3326
描述: Rabbit polyclonal antibody to TNFAIP8L2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 20 KD; 21kD(Calculated).
蛋白号: Q6P589
RRID: AB_2835693

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
TNFAIP8L2 Antibody detects endogenous levels of total TNFAIP8L2.
RRID:
AB_2835693
引用格式: Affinity Biosciences Cat# DF3326, RRID:AB_2835693.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AW610835; FLJ23467; Inflammation factor 20; Inflammation factor protein 20; TIPE2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2; TNFAIP8L2; TP8L2_HUMAN; Tumor necrosis factor alpha induced protein 8 like 2; Tumor necrosis factor alpha-induced protein 8-like protein 2;

抗原和靶标

免疫原:
Uniprot:

Q6P589(TP8L2_HUMAN) >>Visit HPA database.

基因/基因ID:
TNFAIP8L2(79626)
序列:
MESFSSKSLALQAEKKLLSKMAGRSVAHLFIDETSSEVLDELYRVSKEYTHSRPQAQRVIKDLIKVAIKVAVLHRNGSFGPSELALATRFRQKLRQGAMTALSFGEVDFTFEAAVLAGLLTECRDVLLELVEHHLTPKSHGRIRHVFDHFSDPGLLTALYGPDFTQHLGKICDGLRKLLDEGKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
91
Sheep
91
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Negative regulator of Toll-like receptor and T-cell receptor function. Prevents hyperresponsiveness of the immune system and maintains immune homeostasis. Inhibits JUN/AP1 and NF-kappa-B activation. Promotes Fas-induced apoptosis (By similarity).

亚基结构:

May interact with CASP8; however, such result is unclear sincecould not reproduce the interaction with CASP8.

蛋白家族:

The central region was initially thought to constitute a DED (death effector) domain. However, 3D-structure data reveal a previously uncharacterized fold that is different from the predicted fold of a DED (death effector) domain. It consists of a large, hydrophobic central cavity that is poised for cofactor binding.

Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.

文献引用

1). 7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice. International Immunopharmacology, 2021 (PubMed: 33813367) [IF=4.8]

Application: WB    Species: mice    Sample: RAW 264.7 cells

Fig. 8. Effects of DFMG on apoptosis and expression of TIPE2 and TLR4 in LPC-induced RAW 264.7 cells. We used DFMG (0.3 μmol/L) to preincubate RAW264.7 cells for 0.5 h and then stimulated by LPC (1 μg/mL) for 24 h. (A) The rate of apoptosis was detected by Annexin V-FITC/PI kit. (B) The levels of TIPE2 and TLR4 mRNA were measured by RT-qPCR. (C) The expression of TIPE2 and TLR4 protein were measured by Western blot. n = 3, Data are presented as mean ± SD from three independent experiments. **P < 0.01.
2). Tumor necrosis factor α‑induced protein 8‑like 2 contributes to penehyclidine hydrochloride pretreatment against lipopolysaccharide‑induced acute lung injury in a mouse model. Molecular Medicine Reports, 2021 (PubMed: 34468006) [IF=3.4]
3). Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway. SAUDI PHARMACEUTICAL JOURNAL, 2022 (PubMed: 35903524) [IF=3.0]
4). Expression and regulation of tumor necrosis factor-α-induced protein-8-like 2 is associated with acute lung injury induced by myocardial ischemia reperfusion in diabetic rats. Microvascular Research, 2020 (PubMed: 32333940) [IF=2.9]

Application: WB    Species: rat    Sample: lung

Fig. 5.| Protein expression of Bax, Bcl-2, cleaved caspase 3, cleaved caspase 9, JNK, p-JNK, TIPE2 and nuclear NF-κB p65 in lung tissues collected after MIR or sham procedure. (a) Western blotting analysis of protein expression.

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