WTAP Antibody - #DF3282

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产品: WTAP 抗体
货号: DF3282
描述: Rabbit polyclonal antibody to WTAP
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Horse, Sheep, Dog, Chicken, Xenopus
分子量: 44 KD; 44kD(Calculated).
蛋白号: Q15007
RRID: AB_2835650

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Horse(90%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
WTAP Antibody detects endogenous levels of total WTAP.
RRID:
AB_2835650
引用格式: Affinity Biosciences Cat# DF3282, RRID:AB_2835650.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686F20131; Female-lethal(2)D homolog; FL2D_HUMAN; hFL(2)D; KIAA0105; MGC3925; Mum2; OTTHUMP00000017522; OTTHUMP00000017523; PNAS 132; PNAS-132; PNAS132; Pre mRNA splicing regulator WTAP; Pre-mRNA-splicing regulator WTAP; Putative pre mRNA splicing regulator female lethal 2D homolog; Putative pre mRNA splicing regulator female lethal(2D); Putative pre-mRNA splicing regulator female lethal(2D) homolog; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1 associating protein; Wilms tumor 1-associating protein; Wilms' tumor 1 associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WT1-associating protein; wtap;

抗原和靶标

免疫原:
Uniprot:

Q15007(FL2D_HUMAN) >>Visit HPA database.

基因/基因ID:
WTAP(9589)
表达:
Q15007 FL2D_HUMAN:

Ubiquitously expressed.

序列:
MTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLTHQSNDTDSSHDPQEEKAVSGKGNRTVGSRHVQNGLDSSVNVQGSVL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Sheep
100
Horse
90
Dog
90
Xenopus
90
Chicken
90
Zebrafish
78
Pig
0
Bovine
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

细胞定位:

Nucleus speckle. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Interacts with WT1. Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3.

蛋白家族:

Belongs to the fl(2)d family.

文献引用

1). Novel role of FTO in regulation of gut-brain communication via Desulfovibrio fairfieldensis-produced hydrogen sulfide under arsenic exposure. Gut microbes, 2025 (PubMed: 39852343) [IF=12.2]
2). SUMOylation modification of FTO facilitates oxidative damage response of arsenic by IGF2BP3 in an m6A-dependent manner. Journal of hazardous materials, 2024 (PubMed: 38723480) [IF=12.2]
3). Comprehensive Analysis of the Transcriptome-Wide m6A Methylation Modification Difference in Liver Fibrosis Mice by High-Throughput m6A Sequencing. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34869362) [IF=4.6]

Application: WB    Species: Mice    Sample: Liver Fibrosis

FIGURE 7 Verification of m6A methylation level and methylase expression in LF. (A) The m6A methylation level in LF. (B) Semiquantitative analysis of m6A methylation. (C) IGV plots of the WTAP m6A level. (D) IGV plots of the ALKBH5 m6A level. (E) IGV plots of the YTHDF1 m6A level. (F) IGV plots of the WTAP expression level. (G) IGV plots of the ALKBH5 expression level. (H) IGV plots of the YTHDF1 expression level. (I) mRNA expression level of WTAP. (J) mRNA expression level of ALKBH5. (K) mRNA expression level of YTHDF1. (L) Protein expression levels of WTAP. (M) Protein expression levels of ALKBH5. (N) Protein expression levels of YTHDF1. (O) Semiquantitative analysis of WTAP protein. (P) Semiquantitative analysis of YTHDF1 protein. (Q) Semiquantitative analysis of ALKBH5 protein. ## p < 0.01 compared with the control group, # p < 0.05 compared with the control group.
4). m6A-mediated lncRNA MAPKAPK5-AS1 induces apoptosis and suppresses inflammation via regulating miR-146a-3p/SIRT1/NF-κB axis in rheumatoid arthritis. Cell cycle (Georgetown, Tex.), 2023 (PubMed: 38225924) [IF=3.4]
5). Wilms' tumor 1-associated protein aggravates ischemic stroke by promoting M1 polarization of microglia by enhancing PTGS2 mRNA stability in an m6A-dependent manner. Cell biology international, 2025 (PubMed: 39687949) [IF=3.3]
6). SILENCING M 6 A READER YTHDC1 REDUCES INFLAMMATORY RESPONSE IN SEPSIS-INDUCED CARDIOMYOPATHY BY INHIBITING SERPINA3N EXPRESSION. Shock (Augusta, Ga.), 2023 (PubMed: 36877222) [IF=2.7]
7). WTAP-Mediated m6A Modification of TRAIL-DR4 Suppresses MH7A Cell Apoptosis. International journal of rheumatic diseases, 2025 (PubMed: 39797510) [IF=2.4]

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