FOXC1/2 Antibody - #DF3252

Figure 7. NET degradation improves mLV damage. C57BL/6 mice were i.c.v. injected with or without DNase I after IVH. Twenty-four h post-IVH, the meninges were collected for observation. (A) Representative WB images showing the expression of CitH3, VEGFC, LYVE-1, PROX1, FOXC2, and VE-cadherin in the meninges in the Sham, IVH, and IVH+DNase I groups 24 h after IVH. GAPDH was used as an internal reference protein. (B-G) Quantification of CitH3, VEGFC, LYVE-1, PROX1, FOXC2, and VE-cadherin expression (n = 6, one-way ANOVA). (H, I) Relative mRNA levels of FOXC2 and VEGFC in the meninges in the various experimental groups (n = 6). (J, K) The concentrations of VEGFC and FOXC2 in the meninges were quantified by ELISA (n = 6, one-way ANOVA). (L) NO concentrations in the meninges were quantifid in the different groups 24 h after IVH (n = 6, one-way ANOVA). (M) A representative image showing the IF staining of the meninges with anti-LYVE-1 antibodies (red) in the COS. (N) The extent of anti-LYVE-1 antibody staining in the meninges of the COS was quantified and is presented as a percentage of the total area (n = 6, one-way ANOVA). (O) Images showing TUNEL and LYVE-1 staining in the TS of Sham-, IVH-, or DNase I-treated mice were selected as representative examples 24 h after IVH. (P) The number of TUNEL-positive LECs surrounding the TS in the meninges of mice (n = 6, one-way ANOVA). *P < 0.05, **p < 0.01. Data are means ± SEMs.

Fig. 5 Effects of MIc on EMT in CCA cells. KKU-452 cells were chemically induced to undergo EMT (iEMT). (A) The effect of MIc on cell morphology at concentrations of 1 and 2.5 μM was determined. EMT marker expression, including N-cadherin, vimentin, ZEB2, and FOXC1/2, was further evaluated by (B) Western blot (Supplementary Fig. S1 Images of original blot 5B) and (C) immunocytofluorescence analyses. The data shown are representative of two reproducible experiments.

Figure 1. |Upregulation of FOXC1 expression in OSCC tissues. (A) IHC staining of FOXC1 expression in OSCC tissues and adjacent normal tissues. Scale bar, 100 µm.

Figure 1. |Immunostaining for AGGF1 (angiogenic factor with G-patch and FHA domain 1), FOXC2 (forkhead box C2), and E-cad (E-cadherin) in esophageal squamous cell carcinoma and control tissue. ; E, Positive FOXC2 in the cytoplasm of cancer cells (400 magnification)