TFAM Antibody - #DF3232

Fig. 5 Adipose tissue-specific omentin overexpression inhibited WNT5A/Ca2+ signaling pathway and improved mitochondrial biogenesis to ameliorate myocardial injury in HF mice. (A) Serum omentin level of HF patients and healthy subjects. A total of 58 HF patients and 38 healthy subjects were enrolled. (B) The omentin serum content of adipose tissue-specific omentin overexpression by intravenous injection of AAV-omentin and negative control by intravenous injection of AAV-NC (n = 8). (C) The expression of omentin in the inguinal fat of mice with adipose tissue-specific omentin overexpression was detected by immunohistochemistry (n = 4). Scale bar = 200 μm. (D) Representative echocardiographs of mice with AAV-omentin overexpression and the statistical results of (E) LV EF, (F) LV FS, (G) stroke volume, (H) LVPW; d, (I) IVS; d, (J) RWT and (K) LV Mass were presented (n = 8). (L) Representative TTC staining images of heart tissues of mice with AAV-omentin overexpression (n = 6). (M) Representative images of H&E and Masson staining of heart tissues of mice with AAV-omentin overexpression (n = 3), scale bar = 250 μm. (N) The serum content of BNP in mice with AAV-omentin overexpression (n = 8). (O) The serum CK activity in mice with AVV-omentin overexpression (n = 8). (P) The protein expression level of WNT5A, Frizzled2 and p-CAMKII/CAMKII were determined by Western blot. β-actin was used as a loading control (n = 5). (Q) The protein expression level of PGC-1α, NRF1, NRF2 and TFAM were determined by Western blot in mice with AAV-omentin overexpression. β-actin was used as a loading control (n = 5). Values are expressed as the means ± SD. (A) Unpaired Student's two-tailed t-test; (B–Q) One-way ANOVA followed by the Dunnett's post hoc test. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Sham; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CAL.

Figure 3. Effects of BPA on the liver SIRT1/PGC-1α pathway. (A) The relative protein levels of PGC-1α, Nrf2, and SIRT1. (B–D) Values of quantitative analysis (n = 4). (E) Relative mRNA levels of SIRT1, PGC-1α, Nrf1, Nrf2, TNF-α, and IL-1β. (F) Percentage of immunostaining for Sirt1. (G) Immunohistochemistry shows the expression of hepatic SIRT1 (400× magnifications). (H) The relative protein levels of TFAM and (I) the relative protein levels of TNF-α. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.01 vs. control group. ns: no significance.

Figure 4. The effects of MEL on the PGC-1α/TFAM pathway in GA tissues of middle-aged mice a-c. Western blot and qRT-PCR were applied to detect the effects of MEL on PGC-1α/TFAM pathwayrelated protein and mRNA levels, including cytochrome c oxidase subunit 4 (COX4), cystatin C (CYTC), nuclear respiratory factor 1 (NRF-1), mitochondrial transcription factor A (TFAM), p-P38, P38, and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), n=3. Data are manifested as mean ± SD * P