SFRS3 Antibody - #DF3230

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产品: SFRS3 抗体
货号: DF3230
描述: Rabbit polyclonal antibody to SFRS3
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 25 KD; 19kD(Calculated).
蛋白号: P84103
RRID: AB_2835610

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
SFRS3 Antibody detects endogenous levels of total SFRS3.
RRID:
AB_2835610
引用格式: Affinity Biosciences Cat# DF3230, RRID:AB_2835610.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

arginine/serine-rich 3; Pre mRNA splicing factor SRP20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor; Splicing factor arginine/serine rich 20 kD; Splicing factor arginine/serine rich 3; SRp20; SRSF3; SRSF3_HUMAN; X16;

抗原和靶标

免疫原:
Uniprot:

P84103(SRSF3_HUMAN) >>Visit HPA database.

基因/基因ID:
SRSF3(6428)
序列:
MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P84103 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
C6 S-Nitrosylation
C10 S-Nitrosylation
K11 Acetylation
K11 Ubiquitination
Y13 Phosphorylation
K23 Acetylation
K23 Ubiquitination
T24 Phosphorylation
R28 Methylation
Y32 Phosphorylation
Y33 Phosphorylation
R37 Methylation
R57 Methylation
T70 Phosphorylation
S81 Phosphorylation
K85 Acetylation
K85 Sumoylation
K85 Ubiquitination
R90 Methylation
S95 Phosphorylation
R98 Methylation
S108 Phosphorylation
S120 Phosphorylation
S122 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
S128 Phosphorylation
S130 Phosphorylation
S138 Phosphorylation
S140 Phosphorylation
S148 Phosphorylation
S150 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation

研究背景

功能:

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.

翻译修饰:

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.

细胞定位:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Recruited to nuclear speckles following interaction with YTHDC1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with CPSF6. Interacts with RBMY1A1. Interacts with SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites.

蛋白家族:

Belongs to the splicing factor SR family.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

文献引用

1). SRSF3 facilitates replication of influenza A virus via binding and promoting the transport of viral mRNA. Veterinary Microbiology, 2022 (PubMed: 35063826) [IF=2.4]

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