Sonic Hedgehog Recombinant Rabbit mAb - #BF4301

WB 1:1000-1:5000, IHC 1:200-1:2000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human

Monoclonal [ReFirm23751]

Sonic Hedgehog Recombinant Rabbit mAb detects endogenous levels of Sonic Hedgehog.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube,the anterior-posterior limb axis,and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila,this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved,the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly,the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product,restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE),a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome,which is characterized by vertebral defects,anal atresia,tracheoesophageal fistula with esophageal atresia,radial and renal dysplasia,cardiac anomalies,and limb abnormalities. Additionally,mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.