ACTN4 Recombinant Rabbit mAb - #BF4073

WB 1:1000-1:3000, IHC 1:100-1:200, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse, Rat

Monoclonal [ReFirm23520]

ACTN4 Recombinant Rabbit mAb detects endogenous levels of ACTN4.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins,including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells,the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions,where it is involved in binding actin to the membrane. In contrast,skeletal,cardiac,and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies,where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle,alpha actinin isoform which is concentrated in the cytoplasm,and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.