GHRL Recombinant Rabbit mAb - #BF3760

WB 1:1000, IHC 1:200-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse

Monoclonal [ReFirm23207]

GHRL Recombinant Rabbit mAb detects endogenous levels of GHRL.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides,ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty,whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities,including hunger,reward perception via the mesolimbic pathway,gastric acid secretion,gastrointestinal motility,and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake,but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin,including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition,antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.