BRCA1 Recombinant Rabbit mAb - #BF3748

IHC 1:100-1:200, IF/ICC 1:500-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human

Monoclonal [ReFirm23195]

BRCA1 Recombinant Rabbit mAb detects endogenous levels of BRCA1.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability,and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors,DNA damage sensors,and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II,and through the C-terminal domain,also interacts with histone deacetylase complexes. This protein thus plays a role in transcription,DNA repair of double-stranded breaks,and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants,some of which are disease-associated mutations,have been described for this gene,but the full-length natures of only some of these variants has been described. A related pseudogene,which is also located on chromosome 17,has been identified.