MUC16 Recombinant Rabbit mAb - #BF3618

WB 1:1000-1:3000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human

Monoclonal [ReFirm23065]

MUC16 Recombinant Rabbit mAb detects endogenous levels of MUC16.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight,O-glycosylated proteins that play an important role in forming a protective mucous barrier,and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus,a large tandem repeat domain,and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated,while the repeat region contains 156 amino acid repeats unit that are rich in serines,threonines,and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules,leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain,and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier,protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers,with higher expression levels associated with poorer outcomes.