GPX1 Recombinant Rabbit mAb - #BF3592

WB 1:1000-1:3000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Rat

Monoclonal [ReFirm23039]

GPX1 Recombinant Rabbit mAb detects endogenous levels of GPX1.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

The protein encoded by this gene belongs to the glutathione peroxidase family,members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione,and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction,mitochondrial function,and maintenance of thiol redox-balance,therefore,by limiting H2O2 accumulation,glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates,which vary in cellular location and substrate specificity. This isozyme is the most abundant,is ubiquitously expressed and localized in the cytoplasm,and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein,containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon,which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure,designated the Sec insertion sequence (SECIS) element,that is necessary for the recognition of UGA as a Sec codon,rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region,and three alleles with 4,5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21.