WT1 Recombinant Rabbit mAb - #BF3416

WB 1:1000, IHC 1:100-1:200, IF/ICC 1:50
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse

Monoclonal [ReFirm22863]

WT1 Recombinant Rabbit mAb detects endogenous levels of WT1.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline,glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system,and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern,with biallelic,and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants,there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of,and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat,and that this process is tissue-restricted and developmentally regulated.