HMGB2 Antibody - #DF3133

Fig. 2. Negative pressure increased HMGB2 expression and AKT phosphorylation together with chondrocyte proliferation. (A) Heatmap of representative molecules revealed by RNA-seq (n = 3). (B) Representative images of EdU staining of CON and NP groups. (C) qRT-PCR analysis of Akt, P21, Hmgb2, Ki67 and CDK6 expression (n = 5). (D) Western blotting analysis of the expression of related molecules (n = 3). (E) qRT-PCR analysis of Akt, P21, Hmgb2, Ki67 and CDK6 expression in the TMJ condylar cartilage of mice in Sham and BAE groups (n = 6). (F) Representative staining of Ki67, HMGB2 and P-AKT of TMJ condylar cartilage of mice in Sham and BAE groups (n = 6). All data are presented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

Fig. 3. Interference with AKT phosphorylation affected the proliferation-promoting effect of nega- tive pressure on superficial cells derived from TMJ condyles of 3-week-old rats. (A) Western blotting analysis (n = 3) of AKT phosphorylation changes after treatment with LY294002. (B) qRT-PCR analysis (n = 5) and (C–D) western blotting analysis (n = 3) of Ki67, CDK6 and HMGB2 for the treatment of LY294002 in CON and NP groups. (E) Western blot- ting analysis (n = 3) of AKT phosphorylation changes after treatment with LY294002. qRT-PCR analysis (n = 6) (F) and western blotting analysis (n = 3) (G–H) of Ki67, CDK6 and HMGB2 for the treatment of LY294002 in CON and NP groups. All data are pre- sented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.