PKA alpha/beta/gamma CAT Recombinant Rabbit mAb - #BF3143

WB 1:1000-1:3000, IHC 1:200-1:500, IF/ICC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse, Rat

Monoclonal [ReFirm22590]

PKA alpha/beta/gamma CAT Recombinant Rabbit mAb detects endogenous levels of PKA alpha/beta/gamma CAT.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes one of the catalytic subunits of protein kinase A,which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits,in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes,including differentiation,proliferation,and apoptosis. Constitutive activation of this gene caused either by somatic mutations,or genomic duplications of regions that include this gene,have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described,and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.