ATF3 Antibody - #DF3110

Figure 6 Piezo1 increased SLC7A11 in a Ca2+/ATF4-dependent manner and increased SLC15A3 in a Ca2+-dependent manner in macrophages. A) Heatmap of the genes of interest (the same as the 17 genes that were upregulated and the 17 genes that were downregulated by Yoda1 shown in Figure 5) identified by a new RNA-seq analysis of Piezo1fl/fl and Piezo1Lyz2 BMDMs, which were treated with DMSO or Yoda1 (5 µm) for 12 h. B) Levels of Atf4, Atf3, Nrf2 and P53 mRNAs in BMDMs after treatment with DMSO or Yoda1 (n = 6). C,D) Representative Western blots and quantification of the protein levels of ATF4, ATF3, NRF2 and P53 in BMDMs (n = 3). E) Representative images of immunofluorescence staining showing the ATF4 level and location in BMDMs treated with DMSO or Yoda1. F) Protein expression of ATF4 in the cytoplasm and nucleus of BMDMs treated with DMSO or Yoda1. G–I) Representative Western blots and quantification of ATF4 and SLC7A11 protein levels in BMDMs after control or Atf4 siRNA transfection and DMSO or Yoda1 treatment (n = 3). J) Flow cytometry analysis of intracellular Ca2+ levels by Fluo-4 staining in BMDMs from Piezo1fl/fl and Piezo1Lyz2 mice treated with DMSO or Yoda1 for 30 min (n = 5 mice per group). K) Fluo-4 fluorescence signals in BMDMs pretreated with 5 µM BAPTA-AM for 24 h followed by DMSO or 5 µm Yoda1 for 30 min were analyzed via flow cytometry (n = 3). L–O) Representative Western blots and quantification of the protein levels of ATF4, SLC7A11 and SLC15A3 in BMDMs (n = 3). The data in (B and D) were analyzed by unpaired Student's t test. Other data were analyzed by one-way ANOVA, followed by the Bonferroni post hoc correction.

Fig. 5. A) The protein expression levels of ATF3 and ACOX in rat liver of different groups. B) Atf3 relative protein expression level C) Acox2 relative protein expression level (*p < 0.05, **p < 0.01, ***p < 0.001, compare to NAFLD model group, SNK-Q test, n=10).

Fig. 4 The Atf3 + subset identified as a senescent chondrocyte population. (A, B) Gene Ontology(A) enrichment and KEGG analysis(B) for differentially expressed genes in Atf3 + chondrocytes of meniscus. X-axis:–log10(p-value);p