Estrogen Receptor-alpha Recombinant Rabbit mAb - #BF3094

WB 1:1000, IHC 1:200-1:500, IF/ICC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse, Rat

Monoclonal [ReFirm22541]

Estrogen Receptor-alpha Recombinant Rabbit mAb detects endogenous levels of Estrogen Receptor-alpha.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes an estrogen receptor,a ligand-activated transcription factor composed of several domains important for hormone binding,DNA binding,and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function,but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer,endometrial cancer,and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants,but the full-length nature of many of these variants has not been determined.