ZO 1 Recombinant Rabbit mAb - #BF3014

WB 1:500-1:2000, IHC 1:200-1:500, IF/ICC 1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Human, Mouse, Rat

Monoclonal [ReFirm22461]

ZO 1 Recombinant Rabbit mAb detects endogenous levels of ZO 1.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins,and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein,including a postsynaptic density 95,disc-large,zona occludens (PDZ) domain,a Src homology (SH3) domain,a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins,cytosolic proteins,and F-actin,which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.