HARS Antibody - #DF3087

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产品: HARS 抗体
货号: DF3087
描述: Rabbit polyclonal antibody to HARS
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 60/52 KD; 57kD(Calculated).
蛋白号: P12081
RRID: AB_2835470

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 50ul RMB¥ 1250 现货
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MS Report
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
克隆:
Polyclonal
特异性:
HARS Antibody detects endogenous levels of total HARS.
RRID:
AB_2835470
引用格式: Affinity Biosciences Cat# DF3087, RRID:AB_2835470.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cytoplasmic; EC 6.1.1.21; FLJ20491; HARS; HisRS; Histidine tRNA ligase, cytoplasmic; histidine translase; Histidine tRNA ligase; Histidine--tRNA ligase; Histidyl tRNA synthetase; Histidyl-tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN; USH3B;

抗原和靶标

免疫原:
Uniprot:

P12081(HARS1_HUMAN) >>Visit HPA database.

基因/基因ID:
HARS(3035)
表达:
P12081 HARS1_HUMAN:

Brain, heart, liver and kidney.

序列:
MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P12081 作为底物

Site PTM Type Enzyme
A2 Acetylation
K12 Ubiquitination
S27 Phosphorylation
K37 Ubiquitination
K42 Ubiquitination
K51 Ubiquitination
K57 Methylation
K57 Ubiquitination
T58 Phosphorylation
S66 Phosphorylation
K75 Ubiquitination
T94 Phosphorylation
K100 Ubiquitination
K106 Ubiquitination
K112 Acetylation
K112 Ubiquitination
Y115 Phosphorylation
K118 Acetylation
K118 Ubiquitination
S126 Phosphorylation
Y138 Phosphorylation
Y150 Phosphorylation
Y156 Phosphorylation
S229 Phosphorylation
K230 Ubiquitination
K240 Ubiquitination
K243 Ubiquitination
K250 Ubiquitination
K257 Ubiquitination
Y270 Phosphorylation
K288 Ubiquitination
K293 Ubiquitination
S356 Phosphorylation
Y363 Phosphorylation
K373 Ubiquitination
K376 Ubiquitination
S391 Phosphorylation
K403 Ubiquitination
S415 Phosphorylation
K418 Ubiquitination
K426 Ubiquitination
K443 Acetylation
K477 Ubiquitination
K499 Ubiquitination

研究背景

功能:

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Brain, heart, liver and kidney.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the class-II aminoacyl-tRNA synthetase family.

研究领域

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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