产品描述
来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse
预测:
Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)
克隆:
Polyclonal
特异性:
GAK Antibody detects endogenous levels of total GAK.
RRID:
AB_2833481
引用格式: Affinity Biosciences Cat# AF0317, RRID:AB_2833481.
引用格式: Affinity Biosciences Cat# AF0317, RRID:AB_2833481.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
Auxilin 2; Cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
描述:
GAK Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily.
序列:
- O14976 GAK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAPHLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDSPPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEGQEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQACKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Horse
82
Bovine
82
Sheep
82
Dog
82
Rabbit
82
Chicken
73
Zebrafish
55
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14976 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| Y338 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| Y367 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K395 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| Y412 | Phosphorylation | P12931 (SRC) | Uniprot |
| S421 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| R439 | Methylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| Y615 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| K682 | Ubiquitination | Uniprot | |
| K685 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K751 | Ubiquitination | Uniprot | |
| Y764 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T776 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| S785 | Phosphorylation | Uniprot | |
| T794 | Phosphorylation | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| T805 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S826 | Phosphorylation | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot | |
| S1096 | Phosphorylation | Uniprot | |
| S1118 | Phosphorylation | Uniprot | |
| S1130 | Phosphorylation | Uniprot | |
| Y1149 | Phosphorylation | Uniprot | |
| S1151 | Phosphorylation | Uniprot | |
| S1154 | Phosphorylation | Uniprot | |
| S1168 | Phosphorylation | Uniprot | |
| K1172 | Ubiquitination | Uniprot | |
| S1176 | Phosphorylation | Uniprot | |
| S1185 | Phosphorylation | Uniprot | |
| R1192 | Methylation | Uniprot | |
| T1213 | Phosphorylation | Uniprot | |
| K1217 | Ubiquitination | Uniprot | |
| K1219 | Ubiquitination | Uniprot | |
| K1278 | Ubiquitination | Uniprot |
研究背景
功能:
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
细胞定位:
Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Cell junction>Focal adhesion.
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
组织特异性:
Ubiquitous. Highest in testis.
蛋白家族:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
限制条款
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