CLK1 Antibody - #DF3189

(2)  
产品: CLK1 抗体
货号: DF3189
描述: Rabbit polyclonal antibody to CLK1
应用: WB IF/ICC
反应: Human, Mouse
预测: Rabbit, Dog
分子量: 57 KD; 57kD(Calculated).
蛋白号: P49759
RRID: AB_2835412

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Rabbit(88%), Dog(89%)
克隆:
Polyclonal
特异性:
CLK1 Antibody detects endogenous levels of total CLK1.
RRID:
AB_2835412
引用格式: Affinity Biosciences Cat# DF3189, RRID:AB_2835412.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDC like kinase 1; CDC-like kinase 1; CDC28/CDC2 like kinase; CLK; CLK/STY; Clk1; CLK1_HUMAN; Dual specificity protein kinase CLK1; Protein tyrosine kinase STY; STY;

抗原和靶标

免疫原:
Uniprot:

P49759(CLK1_HUMAN) >>Visit HPA database.

基因/基因ID:
CLK1(1195)
表达:
P49759 CLK1_HUMAN:

Endothelial cells.

序列:
MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
89
Rabbit
88
Pig
78
Sheep
78
Bovine
38
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P49759 作为底物

Site PTM Type Enzyme
K15 Ubiquitination
K21 Sumoylation
K21 Ubiquitination
S24 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S37 Phosphorylation
K50 Sumoylation
K50 Ubiquitination
S54 Phosphorylation
Y56 Phosphorylation
S59 Phosphorylation
S61 Phosphorylation
K65 Ubiquitination
Y76 Phosphorylation
Y80 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S104 Phosphorylation
S122 Phosphorylation
T123 Phosphorylation
T138 Phosphorylation
S140 Phosphorylation
S158 Phosphorylation
K174 Ubiquitination
K182 Ubiquitination
K191 Sumoylation
K191 Ubiquitination
T285 Phosphorylation
K290 Ubiquitination
S299 Phosphorylation
Y305 Phosphorylation
K308 Ubiquitination
K322 Sumoylation
S328 Phosphorylation
T330 Phosphorylation
S337 Phosphorylation
T338 Phosphorylation
S341 Phosphorylation
T342 Phosphorylation
Y345 Phosphorylation
K400 Ubiquitination
K464 Ubiquitination
K481 Ubiquitination

翻译修饰 - P49759 作为激酶

Substrate Site Source
P00519 (ABL1) T735 Uniprot
P18031 (PTPN1) S50 Uniprot
P18031 (PTPN1) S242 Uniprot
P18031 (PTPN1) S243 Uniprot
Q96I25 (RBM17) S48 Uniprot
Q96I25 (RBM17) S62 Uniprot
Q96I25 (RBM17) S202 Uniprot
Q96I25 (RBM17) S204 Uniprot
Q96I25 (RBM17) S222 Uniprot
Q96I25 (RBM17) S266 Uniprot
Q96I25 (RBM17) S288 Uniprot
Q96I25 (RBM17) S291 Uniprot

研究背景

功能:

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.

翻译修饰:

Autophosphorylates on all three types of residues.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Endothelial cells.

亚基结构:

Interacts with PPIG and UBL5.

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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