YAP Antibody - #DF3182

Fig. 5 The in vitro uptake and effect of DSPE-PEG-CMP-miR302-EXO on gene expression in H9C2 cells. A Fluorescence images indicated internalization of BMSCs-EVs and DSPE-PEG-CMP-miR302-EXO in H9C2 cells. Scale bar = 50 μm. B Quantitative polymerase chain reaction (qRT-PCR) indicated upregulation of miR302 level in H9C2 cells by DSPE-PEG-CMP-miR302-EXO. Relative values were presented as fold change over control. C The MTT assay showed increased proliferation ratio of H9C2 cells by DSPE-PEG-CMP-miR302-EXO. D–F Western blot analysis of Ki67 and Yap levels displayed increased levels of these proteins in cardiomyocytes after co-incubation with DSPE-PEG-CMP-miR302-EXO. GAPDH served as the internal reference. Relative values were presented as fold change over control. a: Control; b: Model; c: DSPE-PEG-CMP; d: DSPE-PEG-CMP-EXO; e: DSPE-PEG-CMP-miR302-EXO; f: miR302. B, E, F ▲▲p 

Fig. 1. YAP1 knockout aggravates PM2.5-induced lung toxicity and inflammation. WT (YAP1flfl) and YAP1-CKO mice were randomly divided into sham and PM2.5 groups. A and B, The certification of YAP1 conditional knockout in mouse lung tissues; C, Representative YAP1 (red) staining photographs. Nuclei were stained by DAPI (blue); D, Histological images of lung samples exhibited by HE staining in WT mice or YAP1-CKO mice with or without PM2.5 exposure; E, Lung injury scores; F, Representative F4/80 (green) staining photographs, nuclei were stained by DAPI (blue); G, The relative number of F4/80-positive cells was calculated; H, Lung wet/dry mass ratio; I, The protein level of BALF; J and K, Concentrations of TNF-α and IL-6 in lung tissues. The values are shown as the mean ± SD (n = 5),

Fig. 3. YAP1 knockout accelerates ferroptosis in PM2.5-induced lung toxicity. A, Representative fluorescent images of ROS staining; B, Quantification of ROS labeling intensity in the lung; C, Representative TEM images. The red arrow indicates representative mitochondria in WT or YAP1-CKO mouse lungs exposed to PM2.5 or not; D, Immunoblotting detection of SLC7A11, GPX4 and FTH1 expression in mice with the indicated treatment; quantitative results are shown in E-G n = 5; lung content of Fe2+ (H), MDA (I) and GSH (J) measured by the corresponding commercial kit, n = 5. The values are shown as the mean ± SD,

Figure 4 Western blot analysis of control, 0.05 mg and 0.5 mg groups of 21- (A) and 35-day-old (B) rat ovaries. Pictures C, D are the relative grey values analysis of CYP19, YAP, PYAP, LATS2, PLATS2 in each group compared with β-actin. Picture C is 21-day-old rat ovaries and D is 35-day-old rat ovaries. Picture E is the estrogen concentration (pg/mL) in each group of 21- and 35-day-old rat ovaries determined by ELISA.