YAP Antibody - #DF3182

Fig. 5 The in vitro uptake and effect of DSPE-PEG-CMP-miR302-EXO on gene expression in H9C2 cells. A Fluorescence images indicated internalization of BMSCs-EVs and DSPE-PEG-CMP-miR302-EXO in H9C2 cells. Scale bar = 50 μm. B Quantitative polymerase chain reaction (qRT-PCR) indicated upregulation of miR302 level in H9C2 cells by DSPE-PEG-CMP-miR302-EXO. Relative values were presented as fold change over control. C The MTT assay showed increased proliferation ratio of H9C2 cells by DSPE-PEG-CMP-miR302-EXO. D–F Western blot analysis of Ki67 and Yap levels displayed increased levels of these proteins in cardiomyocytes after co-incubation with DSPE-PEG-CMP-miR302-EXO. GAPDH served as the internal reference. Relative values were presented as fold change over control. a: Control; b: Model; c: DSPE-PEG-CMP; d: DSPE-PEG-CMP-EXO; e: DSPE-PEG-CMP-miR302-EXO; f: miR302. B, E, F ▲▲p 

Fig. 4 Hippo/YAP signaling mediates circSTX12-related regulation of BMSCs differentiation. A. Western blot analysis of the expression of proteins involved in adipo-osteogenic pathways in circSTX12 knockdown or knockdown rescue experiment in BMSCs after 7 days’ induction in osteogenic medium. n = 3 biological replicates. B. Immunofluorescence analyses of YAP in BMSCs with circSTX12 knockdown or the control. n = 6 biological replicates. YAP (green) and DAPI (blue). Scale bar = 200 μm. C. ARS and ORO staining and quantification assays results on day 14 in circSTX12 knockdown and the control BMSCs after incubated in adipogenic and osteogenic medium containing 2.5 nM YAP inhibitor or not. n = 6 biological replicates. D. Left panel showed the Western blot results of YAP and p-YAP protein levels in BMSCs transfected with ASO-NC, ASO-circSTX12, OE-MST1, or ASO-circSTX12 + OE-MST1. Right panel showed the histogram result in left panel. n = 3 biological replicates. E. ARS and ORO staining and quantification assays results on day 14 in BMSCs transfected with ASO-NC, ASO-circSTX12, OE-MST1, or ASO-circSTX12 + OE-MST1. n = 6 biological replicates. (F) Left panel showed the Western blot results of YAP and p-YAP protein levels in BMSCs transfected with Vector, OE-circSTX12, SI-MST1, or OE-circSTX12 + SI-MST1. Right panel showed the histogram result in left panel. n = 3 biological replicates. (G) ARS and ORO staining and quantification assays results on day 14 in BMSCs transfected with Vector, OE-circSTX12, SI-MST1, or OE-circSTX12 + SI-MST1. Right panel showed the histogram result in left panel. n = 6 biological replicates. All the data are presented as the mean ± SEM.

Fig. 1. YAP1 knockout aggravates PM2.5-induced lung toxicity and inflammation. WT (YAP1flfl) and YAP1-CKO mice were randomly divided into sham and PM2.5 groups. A and B, The certification of YAP1 conditional knockout in mouse lung tissues; C, Representative YAP1 (red) staining photographs. Nuclei were stained by DAPI (blue); D, Histological images of lung samples exhibited by HE staining in WT mice or YAP1-CKO mice with or without PM2.5 exposure; E, Lung injury scores; F, Representative F4/80 (green) staining photographs, nuclei were stained by DAPI (blue); G, The relative number of F4/80-positive cells was calculated; H, Lung wet/dry mass ratio; I, The protein level of BALF; J and K, Concentrations of TNF-α and IL-6 in lung tissues. The values are shown as the mean ± SD (n = 5),

Fig. 3. YAP1 knockout accelerates ferroptosis in PM2.5-induced lung toxicity. A, Representative fluorescent images of ROS staining; B, Quantification of ROS labeling intensity in the lung; C, Representative TEM images. The red arrow indicates representative mitochondria in WT or YAP1-CKO mouse lungs exposed to PM2.5 or not; D, Immunoblotting detection of SLC7A11, GPX4 and FTH1 expression in mice with the indicated treatment; quantitative results are shown in E-G n = 5; lung content of Fe2+ (H), MDA (I) and GSH (J) measured by the corresponding commercial kit, n = 5. The values are shown as the mean ± SD,

Fig. 6 LINC00525 regulates bladder cancer tumor growth through YAP. A and B. The volume and growth of tumors in the following groups (sh Control, sh-00525, sh-00525 + GA-017). C. Immunohistochemical staining shows the expression of KI67, YAP and pYAPS127 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. Scale bar: 100 μm. D. Relative protein expression of YAP, pYAPS127, downstream targets genes (such as CYR61, CTGF and ANKRD1) and LINC00525 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. E-H. The volume and growth of tumors in the following groups (Control, LINC00525, LINC00525 + Cytochalasin D) and analyzed as in C and D

Figure 4 Western blot analysis of control, 0.05 mg and 0.5 mg groups of 21- (A) and 35-day-old (B) rat ovaries. Pictures C, D are the relative grey values analysis of CYP19, YAP, PYAP, LATS2, PLATS2 in each group compared with β-actin. Picture C is 21-day-old rat ovaries and D is 35-day-old rat ovaries. Picture E is the estrogen concentration (pg/mL) in each group of 21- and 35-day-old rat ovaries determined by ELISA.