产品: TRIM38 抗体
货号: AF0307
描述: Rabbit polyclonal antibody to TRIM38
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 53kDa; 53kD(Calculated).
蛋白号: O00635
RRID: AB_2833471

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(100%)
克隆:
Polyclonal
特异性:
TRIM38 Antibody detects endogenous levels of total TRIM38.
RRID:
AB_2833471
引用格式: Affinity Biosciences Cat# AF0307, RRID:AB_2833471.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gm23; MGC8946; RING finger protein 15; RNF15; Ro/SSA ribonucleoprotein homolog; RORET; RP23 480B19.4; tripartite motif containing 38; Zinc finger protein RoRet;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O00635 TRI38_HUMAN:

Ubiquitous.

描述:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.
序列:
MASTTSTKKMMEEATCSICLSLMTNPVSINCGHSYCHLCITDFFKNPSQKQLRQETFCCPQCRAPFHMDSLRPNKQLGSLIEALKETDQEMSCEEHGEQFHLFCEDEGQLICWRCERAPQHKGHTTALVEDVCQGYKEKLQKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLSRLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLYFDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFEVDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEAGVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Dog
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00635 作为底物

Site PTM Type Enzyme
K50 Ubiquitination
S70 Phosphorylation
K75 Ubiquitination
K122 Ubiquitination
K137 Ubiquitination
K142 Ubiquitination
K148 Ubiquitination
K158 Ubiquitination
K167 Acetylation
K171 Ubiquitination
K184 Ubiquitination
K195 Ubiquitination
K203 Ubiquitination
K222 Ubiquitination
K227 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
K284 Ubiquitination
T319 Phosphorylation
K375 Ubiquitination

研究背景

功能:

E3 ubiquitin-protein ligase. Mediates 'Lys-48'-linked polyubiquitination and proteasomal degradation of the critical TLR adapter TICAM1, inhibiting TLR3-mediated type I interferon signaling.

组织特异性:

Ubiquitous.

文献引用

1). Tripartite motif 38 attenuates cardiac fibrosis after myocardial infarction by suppressing TAK1 activation via TAB2/3 degradation. iScience (PubMed: 35982795) [IF=5.8]

Application: WB    Species: Mouse    Sample: cardiac fibrosis

Figure 2 TRIM38 inhibits the transformation of cardiac fibroblasts to myofibroblasts in vitro (A and B) Representative Western blotting (Left) and quantitative results (Right) of TRIM38 protein expression in NRCFs transfected with AdshTRIM38 or AdTRIM38. Three independent experiments; ∗p < 0.05. (C and D) Representative Western blotting (Left) and quantitative results (Right) of collagen I and α-SMA protein expression in NRCFs transfected with AdshTRIM38 or AdTRIM38 stimulated with angiotensin Ⅱ. Three independent experiments; ∗p < 0.05; ∗∗p < 0.01. (E and F) Representative immunofluorescence staining image of collagen I and α-SMA in NRCFs stimulated with angiotensin Ⅱ or not. NRCFs transfected with AdshTRIM38 or AdTRIM38. Scale bar = 50 μm. (G and H) Migration ability of transfected with AdshTRIM38 or AdTRIM38 derived from indicated NRCFs detected by wound healing assay. Three independent experiments; ∗p < 0.05. The result was means ± S.D. Statistics in A and B was performed using unpaired Student t-test. Statistics in C, D and G, H were performed using a one-way ANOVA.

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