ATM Antibody - #AF4119

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产品: ATM 抗体
货号: AF4119
描述: Rabbit polyclonal antibody to ATM
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q13315
RRID: AB_2835361

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
ATM Antibody detects endogenous levels of total ATM.
RRID:
AB_2835361
引用格式: Affinity Biosciences Cat# AF4119, RRID:AB_2835361.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A-T mutated; A-T mutated homolog; AT mutated; AT1; ATA; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated gene; Ataxia telangiectasia mutated homolog (human); Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; ATM; ATM serine/threonine kinase; ATM_HUMAN; DKFZp781A0353; MGC74674; OTTHUMP00000232981; Serine protein kinase ATM; Serine-protein kinase ATM; Serine/threonine-protein kinase ATM; Tefu; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; Telomere fusion protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human ATM, corresponding to a region within N-terminal amino acids.

基因/基因ID:
ATM(472)
描述:
ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). The brain-protective mechanism of fecal microbiota transplantation from young donor mice in the natural aging process via exosome, gut microbiota, and metabolomics analyses. Pharmacological research, 2024 (PubMed: 39053865) [IF=9.1]
2). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.2]

Application: WB    Species: mouse    Sample: testis

Fig. 7. |SiNPs affected the protein expressions of ATM/p53 and TNF-α/TNFR I-mediated signaling pathways. (A) The protein expressions of ATM, p53, Bcl-2, Bax,TNF-α, TNFR I, Caspase-8, and Caspase-3 were detected in the testis of control group and SiNPs group.
3). Silica nanoparticles cause ovarian dysfunction and fertility decrease in mice via oxidative stress-activated autophagy and apoptosis. Ecotoxicology and environmental safety, 2024 (PubMed: 39303637) [IF=6.2]
4). The activated ATM/p53 pathway promotes autophagy in response to oxidative stress-mediated DNA damage induced by Microcystin-LR in male germ cells. Ecotoxicology and Environmental Safety, 2021 (PubMed: 34715501) [IF=6.2]

Application: WB    Species: Mouse    Sample: GC-1 cells

Fig. 4. Changes of ATM and its downstream proteins in GC-1 cells and mouse testis. Expression and analysis of the related proteins in mouse testis (A) and in GC-1 cells (B). *p < 0.05 vs. the control group; #p < 0.05 vs. the corresponding MC-LR exposure group. All data were expressed as  ± SD (n = 3).
5). Inhibition of checkpoint kinase prevents human oocyte apoptosis induced by chemotherapy and allows enhanced tumour chemotherapeutic efficacy. Human reproduction (Oxford, England), 2023 (PubMed: 37451671) [IF=6.0]

Application: WB    Species: human    Sample:

Figure 3.Cisplatin activates the CHEK-TAp63α pathway in human primordial follicles. (A) H&E staining of human foetal ovaries. (B) p53, p63, and p73 immunostaining in human foetal ovaries. (C) H&E staining of human foetal ovary treated with cisplatin and PBS (Con) and histograms showing analysis of the apoptosis ratio of primordial follicles. (D) Conformational change of TAp63α detected by SDS-PAGE. The original and shifted positions of TAp63α are indicated by black and red arrows, respectively. (E) ChIP analysis of cisplatin-treated human foetal ovaries was performed to interrogate the Puma and Noxa promoter regions with specific antibodies for IgG (control) and p63. (F) Western blots for the ATR/ATM-CHEK pathway. (G) H&E staining and the ratio of apoptotic primordial oocytes in human foetal ovaries treated with cisplatin alone or along with CHEK1 inhibitor (CK1) or CHEK2 inhibitor (CK2). Red arrows indicate healthy primordial follicles, and yellow arrows indicate apoptotic primordial follicles. (H) Analysis of apoptosis-related proteins (BAX, BCL2, γH2AX, NOXA, and PUMA) in each group. (I) H&E staining and the ratio of apoptotic primordial oocytes in each group of adult ovaries. Red arrows indicate healthy primordial follicles, and yellow arrows indicate apoptotic primordial follicles. (J) TUNEL staining of adult ovaries. Yellow arrows indicate apoptotic primordial follicles and red arrows indicate healthy primordial follicles. **P 
6). Fraxetin attenuates DNA damage and inflammation in cisplatin-induced nephrotoxicity via FoxO1 activation. International immunopharmacology, 2025 (PubMed: 39765000) [IF=5.6]
7). Research on the anti-aging mechanisms of Panax ginseng extract in mice: a gut microbiome and metabolomics approach. Frontiers in pharmacology, 2024 (PubMed: 38966558) [IF=5.6]
8). LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability. Aging, 2021 (PubMed: 34740994) [IF=3.9]
9). Usnic Acid Inhibits Proliferation and Migration through ATM Mediated DNA Damage Response in RKO Colorectal Cancer Cell. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2021 (PubMed: 33006536) [IF=2.2]
10). Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis. Frontiers in Cell and Developmental Biology, 2023 (PubMed: 38033870)

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