E2F-2 Antibody - #AF4100

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产品: E2F-2 抗体
货号: AF4100
描述: Rabbit polyclonal antibody to E2F-2
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q14209
RRID: AB_2835358

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
E2F-2 Antibody detects endogenous levels of total E2F-2.
RRID:
AB_2835358
引用格式: Affinity Biosciences Cat# AF4100, RRID:AB_2835358.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

dE2F2; E2F transcription factor 2; E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2;

抗原和靶标

免疫原:

A synthesized peptide derived from human E2F-2, corresponding to a region within the internal amino acids.

基因/基因ID:
E2F2(1870)
描述:
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch. Cell death & disease, 2023 (PubMed: 36797258) [IF=8.1]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 7: The potential mechanism of TCFL5 in mitotic/meiotic switch. A The model shows the potential downstream factors regulated by TCFL5 in the mitotic/meiotic switch. B Effect of siTcfl5 on TCLF5 expression; n ≥ 3; Compared to siNC group, ** means p value 

Application: WB    Species: Mouse    Sample:

Fig. 7: The potential mechanism of TCFL5 in mitotic/meiotic switch. A The model shows the potential downstream factors regulated by TCFL5 in the mitotic/meiotic switch. B Effect of siTcfl5 on TCLF5 expression; n ≥ 3; Compared to siNC group, ** means p value 
2). Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2. Frontiers in pharmacology, 2022 (PubMed: 36052143) [IF=5.6]

Application: WB    Species: Mouse    Sample: hippocampus

FIGURE 4. Effects of MOs on E2F2-regulated MyD88/PI3K pathway in the hippocampus. (A–B) qRT-PCR analysis of gene expression of E2F2 and MyD88 in hippocampus tissues. (C–H) Immunoblot analysis of E2F2, MyD88, p-PI3K, p-AKT and p-NF-κB p65 in hippocampus tissues. Data are expressed as means ± SD (n = 3). ##p < 0.01 vs. control; *p < 0.05; **p < 0.01 vs. Mod.
3). Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3. Thoracic cancer, 2023 (PubMed: 36965165) [IF=2.3]

Application: WB    Species: human    Sample: A549 and AH1299 cells

FIGURE 5 DGKA silencing downregulates the expression of CCND3. (a) Volcano plot showing differentially expressed genes in DGKA-depleted vs. scramble siRNA-transfected A549 cells. (b) Venn diagram showing the number of genes in each indicated set. (c) Enrichment of differentially expressed genes in the pathways is illustrated in a Kyoto Encyclopedia of Genes and Genomes enrichment bubble chart. Y-axis represents the pathways, and the X-axis represents rich ratio. (d) Clustering heatmap of eight deregulated genes affected by loss of DGKA. (e) A549 and H1299 cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by real-time qPCR. ***p 

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