TAF4 Antibody - #AF0305
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0305, RRID:AB_2833469.
展开/折叠
TAF2C; TAF2C1; TAF4A; TAFII 130; TAFII 135; TBP associated factor 4; TAF(II)105; TAF2C2; TAF4b RNA polymerase II, TATA box binding protein (TBP) associated factor, 105kDa; TAFII 105; TAFII105; TAFII105ALPHA; TATA box binding protein (TBP) associated factor 4B; TATA box binding protein (TBP) associated factor, RNA polymerase II, C2, 105kD; Transcription initiation factor TFIID 105 kD subunit; Transcription initiation factor TFIID 105 kDa subunit; Transcription initiation factor TFIID subunit 4B;
抗原和靶标
Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.
- O00268 TAF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGNHVVSGSPAGAAGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPAPPAAKLRPPPEGSAGSCAPVPAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGPGPGPGPGKPAGPGAAQTLNGSAALLNSHHAAAPAVSLVNNGPAALLPLPKPAAPGTVIQTPPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATLARPPGHPAGPPTAAPAVPPPAAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRVVQAAPPAAQTLAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATTSGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFLSTLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRSLPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSSSVQRTAGKTAATVTSALQPPVLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTPQQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASMAGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVSYVSHATQQRLQNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDEQEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKVDCPGPGSGAEGSGPGSVVPGSSGVGTPRQFTRQRITRVNLRDLIFCLENERETSHSLLLYKAFLK
- Q92750 TAF4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPAGLTEPAGAAPPAAVSASGTVTMAPAGALPVRVESTPVALGAVTKAPVSVCVEPTASQPLRSPVGTLVTKVAPVSAPPKVSSGPRLPAPQIVAVKAPNTTTIQFPANLQLPPGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQTSSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLVTSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVKQLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRKKRPLESGIEGLKDNLLASGTSSLTATKQLHRPRITRICLRDLIFCMEQEREMKYSRALYLALLK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00268/Q92750 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S23 | Phosphorylation | Uniprot | |
T47 | Phosphorylation | Uniprot | |
S109 | Phosphorylation | Uniprot | |
T396 | Phosphorylation | Uniprot | |
T409 | Phosphorylation | Uniprot | |
S411 | Phosphorylation | Uniprot | |
R414 | Methylation | Uniprot | |
T415 | Phosphorylation | Uniprot | |
S421 | Phosphorylation | Uniprot | |
R424 | Methylation | Uniprot | |
R435 | Methylation | Uniprot | |
R491 | Methylation | Uniprot | |
R516 | Methylation | Uniprot | |
S528 | O-Glycosylation | Uniprot | |
S528 | Phosphorylation | Uniprot | |
S543 | Phosphorylation | Uniprot | |
T571 | Phosphorylation | Uniprot | |
K611 | Ubiquitination | Uniprot | |
K621 | Ubiquitination | Uniprot | |
K631 | Ubiquitination | Uniprot | |
S648 | Phosphorylation | Uniprot | |
Y652 | Phosphorylation | Uniprot | |
S660 | Phosphorylation | Uniprot | |
R754 | Methylation | Uniprot | |
R769 | Methylation | Uniprot | |
K795 | Acetylation | Uniprot | |
S827 | Phosphorylation | Uniprot | |
S857 | Phosphorylation | Uniprot | |
T862 | Phosphorylation | Uniprot | |
K945 | Ubiquitination | Uniprot | |
S1027 | Phosphorylation | Uniprot | |
S1032 | Phosphorylation | Uniprot | |
S1042 | Phosphorylation | Uniprot | |
T1046 | Phosphorylation | Uniprot | |
T1056 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
R63 | Methylation | Uniprot | |
S64 | Phosphorylation | Uniprot | |
R87 | Methylation | Uniprot | |
Y308 | Phosphorylation | Uniprot | |
S313 | Phosphorylation | Uniprot | |
S314 | Phosphorylation | Uniprot | |
T395 | Phosphorylation | Uniprot | |
S489 | O-Glycosylation | Uniprot | |
T502 | Phosphorylation | Uniprot | |
S516 | Phosphorylation | Uniprot | |
S595 | Phosphorylation | Uniprot | |
K599 | Ubiquitination | Uniprot | |
K670 | Ubiquitination | Uniprot | |
K731 | Ubiquitination | Uniprot | |
K799 | Ubiquitination | Uniprot |
研究背景
Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Nucleus.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with ATF7; the interaction inhibits ATF7-mediated tranactivation.
(Microbial infection) Interacts with SV40 Large T antigen.
Belongs to the TAF4 family.
Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.
Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA).
Nucleus. Cytoplasm.
Note: Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation.
Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription.
Belongs to the TAF4 family.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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