产品: TTK 抗体
货号: AF0102
描述: Rabbit polyclonal antibody to TTK
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 100kDa; 97kD(Calculated).
蛋白号: P33981
RRID: AB_2833280

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
TTK Antibody detects endogenous levels of total TTK.
RRID:
AB_2833280
引用格式: Affinity Biosciences Cat# AF0102, RRID:AB_2833280.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ECSTY kinase; ESK; FLJ38280; hMPS1; Monopolar Spindle 1 Like 1; MPH1; Mps 1; MPS1L1; Phosphotyrosine Picked Threonine Kinase; Phosphotyrosine picked threonine-protein kinase; PYT; RP3-357D13.2; ttk; TTK Protein Kinase; TTK_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P33981 TTK_HUMAN:

Present in rapidly proliferating cell lines.

描述:
TTK a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore.
序列:
MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
75
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P33981 作为底物

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
S7 Phosphorylation
T12 Phosphorylation P33981 (TTK)
S15 Phosphorylation P33981 (TTK)
K19 Methylation
K19 Ubiquitination
K26 Ubiquitination
K28 Sumoylation
K28 Ubiquitination
T33 Phosphorylation P33981 (TTK)
S37 Phosphorylation P33981 (TTK)
T46 Phosphorylation
K71 Sumoylation
K74 Ubiquitination
S80 Phosphorylation P33981 (TTK)
K86 Ubiquitination
K102 Ubiquitination
S108 Phosphorylation
K120 Ubiquitination
Y131 Phosphorylation
K140 Ubiquitination
K163 Ubiquitination
K168 Ubiquitination
K191 Ubiquitination
K192 Ubiquitination
K201 Ubiquitination
T210 Phosphorylation
S214 Phosphorylation
S216 Phosphorylation
S218 Phosphorylation
Y255 Phosphorylation
S258 Phosphorylation
K266 Ubiquitination
S281 Phosphorylation P15056 (BRAF)
K287 Sumoylation
K287 Ubiquitination
T288 Phosphorylation O96017 (CHEK2) , P33981 (TTK)
S291 Phosphorylation
K315 Ubiquitination
S317 Phosphorylation
S321 Phosphorylation
K328 Ubiquitination
S333 Phosphorylation
K336 Ubiquitination
S345 Phosphorylation
T351 Phosphorylation
S353 Phosphorylation
K359 Ubiquitination
T360 Phosphorylation P33981 (TTK)
S362 Phosphorylation P33981 (TTK)
S363 Phosphorylation P33981 (TTK)
K367 Sumoylation
K367 Ubiquitination
T371 Phosphorylation
K372 Ubiquitination
Y374 Phosphorylation
S382 Phosphorylation
K385 Methylation
K385 Ubiquitination
K392 Ubiquitination
S393 Phosphorylation
K415 Ubiquitination
S433 Phosphorylation
K434 Ubiquitination
S436 Phosphorylation P15056 (BRAF) , P33981 (TTK)
S440 Phosphorylation
K443 Ubiquitination
K448 Ubiquitination
K452 Ubiquitination
T453 Phosphorylation
S455 Phosphorylation
T458 Phosphorylation
Y462 Phosphorylation
S464 Phosphorylation
R467 Methylation
T468 Phosphorylation
K472 Sumoylation
K529 Ubiquitination
K538 Ubiquitination
K546 Ubiquitination
K547 Ubiquitination
K553 Ubiquitination
T564 Phosphorylation P33981 (TTK)
K577 Ubiquitination
S582 Phosphorylation P33981 (TTK)
K584 Ubiquitination
T594 Phosphorylation
K617 Ubiquitination
S618 Phosphorylation
K625 Ubiquitination
K649 Ubiquitination
T675 Phosphorylation P33981 (TTK)
T676 Phosphorylation P33981 (TTK)
S677 Phosphorylation P33981 (TTK)
K680 Ubiquitination
S682 Phosphorylation
T686 Phosphorylation P33981 (TTK)
K696 Ubiquitination
K708 Methylation
S742 Phosphorylation P33981 (TTK)
K762 Ubiquitination
K769 Ubiquitination
T795 Phosphorylation P33981 (TTK)
K803 Ubiquitination
T805 Phosphorylation P33981 (TTK)
T806 Phosphorylation P33981 (TTK)
K810 Ubiquitination
Y811 Phosphorylation P33981 (TTK)
S821 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P33981 (TTK)
S824 Phosphorylation P33981 (TTK)
K827 Ubiquitination
K830 Ubiquitination
Y833 Phosphorylation
Y836 Phosphorylation
S837 Phosphorylation
S845 Phosphorylation P33981 (TTK)
S847 Phosphorylation P33981 (TTK)
K848 Ubiquitination
T849 Phosphorylation P33981 (TTK)
K852 Acetylation
K853 Acetylation

翻译修饰 - P33981 作为激酶

Substrate Site Source
O60566 (BUB1B) S435 Uniprot
O60566 (BUB1B) S543 Uniprot
O60566 (BUB1B) S670 Uniprot
O60566 (BUB1B) S1043 Uniprot
O96017 (CHEK2) T68 Uniprot
P00519 (ABL1) T735 Uniprot
P04637 (TP53) T18 Uniprot
P07900 (HSP90AA1) T115 Uniprot
P33981 (TTK) T12 Uniprot
P33981 (TTK) S15 Uniprot
P33981 (TTK) T33 Uniprot
P33981 (TTK) S37 Uniprot
P33981 (TTK) S80 Uniprot
P33981 (TTK) T288 Uniprot
P33981 (TTK) T360 Uniprot
P33981 (TTK) S362 Uniprot
P33981 (TTK) S363 Uniprot
P33981 (TTK) S436 Uniprot
P33981 (TTK) T564 Uniprot
P33981 (TTK) S582 Uniprot
P33981 (TTK) T675 Uniprot
P33981 (TTK) T676 Uniprot
P33981 (TTK) S677 Uniprot
P33981 (TTK) T686 Uniprot
P33981 (TTK) S742 Uniprot
P33981 (TTK) T795 Uniprot
P33981 (TTK) T805 Uniprot
P33981 (TTK) T806 Uniprot
P33981 (TTK) Y811 Uniprot
P33981 (TTK) S821 Uniprot
P33981 (TTK) S824 Uniprot
P33981 (TTK) S845 Uniprot
P33981 (TTK) S847 Uniprot
P33981 (TTK) T849 Uniprot
P38646 (HSPA9) T62 Uniprot
P38646 (HSPA9) S65 Uniprot
P54132 (BLM) S144 Uniprot
P54132 (BLM) S1290 Uniprot
P54132 (BLM) S1296 Uniprot
Q53HL2 (CDCA8) T88 Uniprot
Q53HL2 (CDCA8) T94 Uniprot
Q53HL2 (CDCA8) T169 Uniprot
Q53HL2 (CDCA8) T230 Uniprot
Q53HL2 (CDCA8) S238 Uniprot
Q96E14 (RMI2) S112 Uniprot

研究背景

功能:

Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

组织特异性:

Present in rapidly proliferating cell lines.

亚基结构:

Interacts with TPR; the interactions occurs in a microtubule-independent manner.

蛋白家族:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

文献引用

1). Liu K et al. Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis. International Journal of Molecular Sciences 2023 Mar 27;24(7) (PubMed: 37047268) [IF=5.6]

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