产品: HMGA2 抗体
货号: AF4069
描述: Rabbit polyclonal antibody to HMGA2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Sheep
分子量: 18kd; 12kD(Calculated).
蛋白号: P52926
RRID: AB_2835351

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(80%), Bovine(%), Sheep(%)
克隆:
Polyclonal
特异性:
HMGA2 Antibody detects endogenous levels of total HMGA2.
RRID:
AB_2835351
引用格式: Affinity Biosciences Cat# AF4069, RRID:AB_2835351.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HMGA 2; BABL; High mobility group (nonhistone chromosomal) protein isoform I C; High mobility group (nonhistone chromosomal) protein isoform IC; High Mobility Group AT hook 2; High Mobility Group AT hook protein 2; High mobility group AT-hook protein 2; High mobility group protein HMGI C; High mobility group protein HMGI-C; High mobility group protein HMGIC; High mobility group protein I, isoform C; HMGA2; HMGA2_HUMAN; HMGI C; HMGIC; LIPO; Non histone chromosomal architectural protein HMGI C; Non histone chromosomal architectural protein HMGIC; Pygmy; STQTL9;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
序列:
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Pig
80
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity).

翻译修饰:

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2 (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with E4F1. Interacts with NEK2 (By similarity).

蛋白家族:

Belongs to the HMGA family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis. Cancer Cell International, 2021 (PubMed: 34281530) [IF=5.8]

Application: WB    Species: Human    Sample: MDA-MB-453, MDA-MB-231 and MCF-10A cells

Fig. 5 The relationship of miR-216b and HMGA2. A The binding sequence of miR-216b and HMGA2. B, C Luciferase activity was examined in cells with co-transfection of HMGA2-WT or HMGA2-MUT and miR-216b mimic or miR-con. D HMGA2 abundance in cells with transfection of biotin-miR-con or biotin-miR-216b after RNA pull-down. E HMGA2 protein expression in MDA-MB-453, MDA-MB-231 and MCF-10A cells. F HMGA2 abundance in 5-FU-resistant and sensitive MDA-MB-231 and MDA-MB-453 cells. G, H HMGA2 protein level in cells with transfection of miR-con, miR-216b mimic, in-miR-con or in-miR-216b. Mock: non-transfected group. * P < 0.05

2). Long non-coding RNA LINC00460 promotes proliferation and inhibits apoptosis of cervical cancer cells by targeting microRNA-503-5p. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32740791) [IF=3.5]

Application: WB    Species: human    Sample: SiHa and HeLa cells

Fig. 4 | LINC00460 acts as a competing endogenous RNA of miR503-5p in SiHa and HeLa cells.d–h Expressions of AKT2,HMGA2 and SHOX2 in SiHa and HeLa cells by western blotting.**p<0.01 versus cells co-transfected with wild-type LINC00460 and miR-503-5p mimic. ##p<0.01 versus cells transfected with NC shRNA. WT wild-type LINC00460, MUT mutated LINC00460

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