FOXP4 Antibody - #AF4057

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产品: FOXP4 抗体
货号: AF4057
描述: Rabbit polyclonal antibody to FOXP4
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 74kd; 73kD(Calculated).
蛋白号: Q8IVH2
RRID: AB_2835349

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
FOXP4 Antibody detects endogenous levels of total FOXP4.
RRID:
AB_2835349
引用格式: Affinity Biosciences Cat# AF4057, RRID:AB_2835349.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1200010K03Rik; 2310007G05Rik; FKHLA; FLJ40908; FLJ44184; Fork head related protein like A; Fork head-related protein-like A; Forkhead box P4; Forkhead box protein P4; FOX P4; FOXP 4; foxp4; FOXP4_HUMAN; hFKHLA; mFKHLA; OTTHUMP00000016374; OTTHUMP00000039784; OTTHUMP00000043412; OTTHUMP00000043536; Winged helix repressor FOX P4; Winged helix repressor FOXP 4; Winged helix repressor FOXP4;

抗原和靶标

免疫原:
Uniprot:

Q8IVH2(FOXP4_HUMAN) >>Visit HPA database.

基因/基因ID:
FOXP4(116113)
描述:
Transcriptional repressor that represses lung-specific expression.
序列:
MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQALQVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQQLQEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASGPLQTLPQAAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQPTVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
58
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Transcriptional repressor that represses lung-specific expression.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms homodimers and heterodimers with FOXP1 and FOXP2. Dimerization is required for DNA-binding (By similarity).

蛋白家族:

The leucine-zipper is required for dimerization and transcriptional repression.

文献引用

1). Downregulation of microRNA‑423‑5p suppresses TGF‑β1‑induced EMT by targeting FOXP4 in airway fibrosis. Molecular Medicine Reports, 2022 (PubMed: 35642665) [IF=3.4]

Application: IF/ICC    Species:    Sample:

Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑ cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay. (E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR. The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. * P<0.05 and **P<0.01. miR, microRNA; FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.

Application: WB    Species: Human    Sample: BEAS‑2B cell

Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑ cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay. (E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR. The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. * P<0.05 and **P<0.01. miR, microRNA; FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.

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